Results 261-270 of about 1,000
- Genome Editing, Transcriptional Regulation, and Forward Genetic Screening Using CRISPR-Cas12a Systems in Yarrowia lipoly... new windowClass II Type V endonucleases have increasingly been adapted to develop sophisticated and easily accessible synthetic biology tools for genome editing, transcriptional regulation, and functional genomic screening in a wide range of organisms. One
Date: (2024) Authors: Adithya Ramesh , Sangcheon Lee , Ian Wheeldon Ref: Synthetic Biology Authors: " Pierre-François Perroud","Anouchka Guyon-Debast ","Josep M Casacuberta","Wyatt Paul","Jean-Philippe Pichon","David Comeau","Fabien Nogué", - Targeted Gene Editing of Nuclear-Encoded Plastid Proteins in Phaeodactylum tricornutum via CRISPR/Cas9 new windowGenome modifications in microalgae have emerged as a crucial and indispensable tool for research in fundamental and applied biology. In particular, CRISPR/Cas9 has gained significant recognition as a highly effective method for genome engineering in
Date: (2024) Authors: Cécile Giustini , Jhoanell Angulo , Florence Courtois , Guillaume Allorent Ref: Plastids - CRISPR-array-mediated imaging of non-repetitive and multiplex genomic loci in living cells new windowDynamic imaging of genomic loci is key for understanding gene regulation, but methods for imaging genomes, in particular non-repetitive DNAs, are limited. We developed CRISPRdelight, a DNA-labeling system based on endonuclease-deficient CRISPR–Cas12a
Date: (2024) Authors: Liang-Zhong Yang , Yi-Hui Min , Yu-Xin Liu , Bao-Qing Gao ... Ling-Ling Chen - Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast. new window
Date: 2025-01-01 Authors: Ishikawa K, Saitoh S. Journal: Methods in molecular biology (Clifton, N.J.) - scCUTseq is a versatile framework for spatially resolved single-cell genomic analyses of intratumor heterogeneity\n new window
Authors: "Ning Zhang","Michele Simonetti","Luuk Harbers","Thomas Helleday","Vassilis Roukos","Caterina Marchio","Magda Bienko","Nicola Crosetto", Authors: "Daniel A Kuppers","Patrick Paddison", - Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development new windowNumerous toxins threaten humans, but specific antidotes are unavailable for most of them. Although CRISPR screening has aided the discovery of the mechanisms of some toxins, developing targeted antidotes remains a significant challenge. Recently, we
Date: (2024) Authors: Bei Wang , Yu Xu , Arabella H. Wan , Guohui Wan , Qiao-Ping Wang Authors: "Dan Dou","C. Alexander Boecker","Erika L.F. Holzbaur", - The CRISPR/Cas9 system is a revolutionary technology for genome editing that allows for precise and efficient modifications of DNA sequences. The system is composed of two main components, the Cas9 enzyme and a guide RNA (gRNA). The gRNA is designed
Date: (2024) Authors: Shahroz Rahman , Abdul Rehman Ikram , Farrukh Azeem , Muhammad Tahir ul Qamar ... Mehboob-ur-Rahman Ref: Plant Functional Genomics