Results 361-370 of about 1,000
- A protocol for the detection of precision genome editing in human cells using One-pot DTECT. new window
Date: 2024-09-16 Authors: Langley J, Baudrier L, Billon P. Journal: STAR protocols - Transgenesis technologies, such as overexpression or RNA interference-mediated suppression, have often been used to alter the activity of target genes. More recently developed targeted genome modification methods using customizable endonucleases
Date: (2024) Authors: Hiroshi Hisano , Robert E. Hoffie , Jochen Kumlehn , Kazuhiro Sato Ref: Seed Dormancy - Protocol to identify receptors of secreted proteins through CRISPR-Cas9 whole-genome screening technology. new window
Date: 2024-09-14 Authors: Hu X, Wang Y. Journal: STAR protocols - Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated reverse transcription and integration of the de novo synthesized single-stranded DNA. To circumvent one of the main bottlenecks of the method, the competition of
Date: (2024) Authors: Dong-Jiunn Jeffery Truong , Julian Geilenkeuser , Stephanie Victoria Wendel , Julius Clemens Wilming ... Gil Gregor Westmeyer Date: 2024-09-12 Authors: Noguchi Y, Maruoka M, Suzuki J. Journal: STAR protocols - Post-translational modification-centric base editor screens to assess phosphorylation site functionality in high through... new windowSignaling pathways that drive gene expression are typically depicted as having a dozen or so landmark phosphorylation and transcriptional events. In reality, thousands of dynamic post-translational modifications (PTMs) orchestrate nearly every
Date: (2024) Authors: Patrick H. Kennedy , Amin Alborzian Deh Sheikh , Matthew Balakar , Alexander C. Jones ... Samuel A. Myers - Protocol for CRISPR-Cas12a genome editing of protein tyrosine phosphatases in human pluripotent stem cells and functiona... new window
Date: 2024-09-06 Authors: Negueruela J, Vandenbempt V, Talamantes S, Ribeiro-Costa F, Nunes M, Dias A, Bansal M, Gurzov EN. Journal: STAR protocols - Preamplification-free viral RNA diagnostics with single-nucleotide resolution using MARVE, an origami paper-based colori... new windowThe evolution and mutation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are urgent concerns as they pose the risk of vaccine failure and increased viral transmission. However, affordable and scalable tools allowing rapid
Date: (2024) Authors: Ting Zhang , Yuxi Wang , Xucong Teng , Ruijie Deng , Jinghong Li - Expression and purification of cell-penetrating Cas9 and Cas12a enzymes for peptide-assisted genome editing. new window
Date: 2024-09-05 Authors: Cuomo RG, Zhang Z, Yamada K, Krosky AJ, Shi J, Kohli RM, Parker JB. Journal: Methods in enzymology - Viral metagenomics is one of the most widely used approaches to study viral population genomics. With the recent development of bioinformatic tools, the number of molecular biological methods, programs, and software to analyze viral metagenome data
Date: (2024) Authors: Kira Moon , Jang-Cheon Cho Ref: Viral Metagenomics