Results 381-390 of about 1,000
Date: 2024-08-16 Authors: Kongkaew R, Uttamapinant C, Patchsung M. Journal: Journal of visualized experiments : JoVE - Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human t... new windowPseudouridine (Ψ) is one of the most abundant modifications in cellular RNA. However, its function remains elusive, mainly due to the lack of highly sensitive and accurate detection methods. Here, we introduced 2-bromoacrylamide-assisted cyclization
Date: (2024) Authors: Haiqi Xu , Linzhen Kong , Jingfei Cheng , Khatoun Al Moussawi ... Chun-Xiao Song Date: 2024-08-14 Authors: Lin TY, Chien SH, Pon LA, Liao PC. Journal: Methods in enzymology - Multiplex Immunofluorescence Staining Protocol for the Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fix... new windowHypoxia is a common condition in rapidly proliferating tumors and occurs when oxygen delivery to the tissue is scarce. It is a prevalent feature in ~90% of solid tumors. The family of HIF (hypoxia-inducible factor) proteins—HIF1α and HIF2α—are the
Date: (2024) Authors: Harsh H. Oza , Daniele M. Gilkes Ref: Hypoxia Date: 2024-08-14 Authors: Yan Y, Schetelig MF. Journal: STAR protocols - Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Prot... new windowImmunofluorescence (IF) microscopy is arguably one of the most commonly used methods for studying structure and composition of stress granules (SGs). While in most cases standard IF protocols are sufficient to visualize protein components of SGs,
Date: (2024) Authors: Jakub Kochan , Mateusz Wawro Ref: Single Cell Analysis - Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research. new window
Date: 2024-08-13 Authors: Casabianca A, Bellina M, Dudgeon C, Nezhad MS, Harris C, Fiore S, Bernet A, Narrow W, Prela O, Wang L, Astsaturov I, Mehlen P, Carpizo DR. Journal: STAR protocols - Macrophages contribute to many aspects of development and homeostasis, innate and acquired immunity, immunopathology, and tissue repair. Every tissue contains an abundant resident macrophage population. Inflammatory stimuli promote the recruitment of
Date: (2024) Authors: Stephen Huang , Dylan Carter-Cusack , Emma Maxwell , Omkar L. Patkar ... David A. Hume Ref: Tissue-Resident Macrophages - Protocol for establishing inducible CRISPRd system for blocking transcription factor-binding sites in human pluripotent ... new window
Date: 2024-08-11 Authors: Matsui S, Shiley JR, Buckley M, Lim HW, Hu YC, Mayhew CN, Iwafuchi M. Journal: STAR protocols - Positive selection screens are high-throughput assays to characterize novel enzymes from environmental samples and enrich for more powerful variants from libraries in applications such as biodiversity mining and directed evolution. However, overly
Date: (2024) Authors: Michael A. Mechikoff , Kok Zhi Lee , Kevin V. Solomon Ref: Synthetic Biology