Results 431-440 of about 1,000
- Genome editing has revolutionized plant research and plant breeding by enabling precise genome manipulation. In particular, the application of type II CRISPR-Cas9 systems to genome editing has proved an important milestone, accelerating genetic
Date: (2023) Authors: Naoki Wada , Keishi Osakabe , Yuriko Osakabe Ref: Plant Genome Engineering - CRISPR-Cas9 editing of synaptic genes in human embryonic stem cells for functional analysis in induced human neurons new window
Date: 2024-05-24 Authors: Houcek A, Ma Z, Trauterman B, Uzay B, Monteggia L, Kavalali E. Journal: STAR protocols - The CRISPR/Cas system has emerged as a versatile platform for sequence-specific genome engineering in plants. Beyond genome editing, CRISPR/Cas systems, based on nuclease-deficient Cas9 (dCas9), have been repurposed as an RNA-guided platform for
Date: (2023) Authors: Changtian Pan , Yiping Qi Ref: Plant Gene Regulatory Networks - Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes new windowThe clustered regularly interspaced short palindromic repeats (CRISPR) technology has made it possible to produce genome-edited (GE) animals more easily and rapidly than before. In most cases, GE mice are produced by microinjection (MI) or by in
Date: (2023) Authors: Masahiro Sato , Ayaka Nakamura , Marie Sekiguchi , Takashi Matsuwaki ... Masato Ohtsuka Ref: Transgenesis - Protocol for scarless genome editing of human pluripotent stem cell based on orthogonal selective reporters. new window
Date: 2024-05-23 Authors: Zhao Y, Pan Z, Hong Z, Sun M, Hong Y, Peng X, Li X, Wang X, Wang K. Journal: STAR protocols - Genome editing technology has facilitated the studies on exploring specific gene functions in diverse living organisms. The technology has also contributed to creating high-value livestock in industry fields in terms of enhancing productivity or
Date: (2023) Authors: Jae Yong Han , Hong Jo Lee Ref: Genome Editing in Animals - Protocol for mass spectrometric profiling of lysine malonylation by lysine acetyltransferase in CRISPRi K562 cell lines. new window
Date: 2024-05-20 Authors: Zhang R, Bons J, Rose JP, Schilling B, Verdin E. Journal: STAR protocols - Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector new windowThe clustered regularly interspersed short palindromic repeats (CRISPR)/Cas systems have become the most widely adopted genome editing platform owing to their unprecedented simplicity, efficiency, and versatility. Typically, the genome editing enzyme
Date: (2023) Authors: Xiaonan Ma , Xuemei Li , Zhenghe Li Ref: Plant Genome Engineering - Protocol to efficiently induce CRISPR-Kill-mediated cell ablation in Arabidopsis thaliana. new window
Date: 2024-05-19 Authors: Gehrke F, Wolf S, Puchta H. Journal: STAR protocols - Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy new windowThis protocol describes a novel approach harnessing the technology of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9-based gene editing for treating retinal angiogenesis. In this system, adeno-associated
Date: (2023) Authors: Wenyi Wu , Hetian Lei Ref: Diabetic Retinopathy