Results 611-620 of about 1,000
- Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq new windowCRISPR-Cas systems consist of a complex ribonucleoprotein (RNP) machinery encoded in prokaryotic genomes to confer adaptive immunity against foreign mobile genetic elements. Of these, especially the class 2, Type II CRISPR-Cas9 RNA-guided systems
Date: (2022) Authors: Sahil Sharma , Cynthia M. Sharma Ref: Post-Transcriptional Gene Regulation - Quantitative Measurement of Tau Aggregation in Genetically Modified Rats with Neurodegeneration. new window
Date: 2024-01-01 Authors: Lee Y, Morrow EM. Journal: Methods in molecular biology (Clifton, N.J.) - First identified as a viral defense mechanism, clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) has been transformed into a gene-editing tool. It now affords promise in the treatment and
Date: (2022) Authors: Jonathan Herskovitz , Mahmudul Hasan , Milankumar Patel , Bhavesh D. Kevadiya , Howard E. Gendelman Ref: HIV Reservoirs - Targeted Modification of Epigenetic Marks Using CRISPR/dCas9-SunTag-Based Modular Epigenetic Toolkit. new window
Date: 2024-01-01 Authors: Song MK, Kim YS. Journal: Methods in molecular biology (Clifton, N.J.) - CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration new windowClustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-associated system) has become the multipurpose tool to manipulate plant genome via their programmable sequence recognition, binding, and cleavage activities. Efficient
Date: (2022) Authors: Raviraj Banakar , Krishan M. Rai , Feng Zhang Ref: Protoplast Technology - Efficient Generation of CRISPR/Cas9-Mediated Knockout Human Primary Keratinocytes by Electroporation. new window
Date: 2024-01-01 Authors: Karakaya T, Slaufova M, Di Filippo M, Hennig P, Fenini G, Kündig T, Beer HD. Journal: Methods in molecular biology (Clifton, N.J.) - Protoplast transfection is widely used in plant research to rapidly evaluate RNA degradation, reporter assay, gene expression, subcellular localization, and protein-protein interactions. In order to successfully use protoplast transfection with the
Date: (2022) Authors: Fu-Hui Wu , Yu-Hsuan Yuan , Chen-Tran Hsu , Qiao-Wei Cheng , Choun-Sea Lin Ref: Protoplast Technology - Gene editing is increasing its popularity day by day especially as an essential tool for the research. It is based on two recombination mechanisms in mammalian cells: nonhomologous end-joining (NHEJ) and homology-directed repair (HDR). The first one
Date: (2022) Authors: Luca Falato , Birgit Vunk , Ülo Langel Ref: Cell Penetrating Peptides Date: 2024-01-01 Authors: Wu H, Chen LL. Journal: Methods in molecular biology (Clifton, N.J.) - Engineering Modular Polyketide Biosynthesis in Streptomyces Using CRISPR/Cas: A Practical Guide new windowThe CRISPR/Cas system, which has been widely applied to organisms ranging from microbes to animals, is currently being adapted for use in Streptomyces bacteria. In this case, it is notably applied to rationally modify the biosynthetic pathways giving
Date: (2022) Authors: Jean-Malo Massicard , Li Su , Christophe Jacob , Kira J. Weissman Ref: Engineering Natural Product Biosynthesis