Results 661-670 of about 1,000
- CRISPR-Cas9 Editing of the Synthesis of Biodegradable Polyesters Polyhydroxyalkanaotes (PHA) in Pseudomonas putida KT2440 new windowGenome editing technologies allow us to study the metabolic pathways of cells and the contribution of each associated enzyme to various processes, including polyhydroxyalkanoate (PHA) synthesis. These biodegradable polyesters accumulated by a range
Date: (2022) Authors: Si Liu , Tanja Narancic , Chris Davis , Kevin E. O’Connor Ref: Enzyme Engineering - Cas9-mediated genome editing is one tool investigators can use to study fungal pathogens. Such methodologies allow the investigator to examine how fungal cells differ from human cells and thus potentially identify novel therapeutic targets. In this
Date: (2022) Authors: Ben A. Evans , Douglas A. Bernstein Ref: Candida Species - With the establishment of the CRISPR-Cas9 molecular tool as a DNA editing system in 2012, the handling of gene editing experiments was strongly facilitated pushing reverse genetics approaches forward in many organisms. These new gene editing
Date: (2022) Authors: Simon Kelterborn , Francisca Boehning , Irina Sizova , Olga Baidukova ... Peter Hegemann Ref: Plant Synthetic Biology Date: 2024-01-01 Authors: Bornhorst D, Gheller B, Zon LI. Journal: Methods in molecular biology (Clifton, N.J.) - The CRISPR/Cas9 system provides the means to make precise and purposeful modifications to the genome via homology-directed repair (HDR). In Drosophila, a wide variety of tools provide flexibility to achieve these ends. Here, we detail a method to
Date: (2022) Authors: Kevin G. Nyberg , Richard W. Carthew Ref: Drosophila - Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention new windowIntron retention (IR) regulates gene expression to control fundamental biological processes like metabolism, differentiation, and cell cycle. Despite a wide variety of genes controlled by IR, few techniques are available to identify regulators of IR
Date: (2022) Authors: Anna M. Scarborough , Ashwin Govindan , Nicholas K. Conrad Ref: Alternative Splicing - Efficient Generation of CRISPR /Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Acce... new windowInvestigating the process of gamete formation in plants often requires the use of mutants of selected genes in various genetic backgrounds. For example, analysis of meiotic recombination based on sequencing or genotyping requires the generation of
Date: (2022) Authors: Tomasz Bieluszewski , Maja Szymanska-Lejman , Wojciech Dziegielewski , Longfei Zhu , Piotr A. Ziolkowski Ref: Plant Gametogenesis - The ability to interrogate gene function in Plasmodium parasites has been greatly enhanced by the advent of CRISPR/Cas9 systems. The breadth of genome manipulations ranges from single point mutations to large multigene deletions, however many of the
Date: (2022) Authors: Sophie Adjalley , Marcus Chee Lee Ref: Malaria Immunology - CRISPR Engineering of Bacteriophage T4 to Design Vaccines Against SARS-CoV-2 and Emerging Pathogens new windowThe COVID-19 pandemic brought to the fore the urgent need for vaccine design and delivery platforms that can be rapidly deployed for manufacture and distribution. Though the mRNA and adenoviral vector platforms have been enormously successful to
Date: (2022) Authors: Jingen Zhu , Neeti Ananthaswamy , Swati Jain , Himanshu Batra ... Venigalla B. Rao Ref: Vaccine Design - Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. new window
Date: 2023-12-13 Authors: Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Journal: STAR protocols