Results 671-680 of about 1,000
- Genome-wide CRISPR and siRNA screening methodologies are powerful tools that are aptly suited to the discovery of essential genes. In this chapter, we outline our methods to conduct sequential CRISPR and siRNA screens to quickly and efficiently
Date: (2022) Authors: Luke DeHart , Oliver P. Yockey , Jesse Bakke Ref: Essential Genes and Genomes Date: 2023-12-12 Authors: Yan D, Xue J, Xiao J, Lyu Z, Yang X. Journal: STAR protocols - Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases new windowFor the diagnosis of pathogens, amplification and detection of their genetic material is an essential step, but the traditional method of amplification and detection requires sophisticated equipment or complex experimental procedures and is not
Date: (2022) Authors: Ajay Kumar Singh , Soumen Naskar , Pramod W. Ramteke , Rohit Kumar Ref: Protocols for the Diagnosis of Pig Viral Diseases - CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics. new window
Date: 2023-12-08 Authors: Van Heest AE, Deng F, Zhao RT, Harzallah NS, Fleming HE, Bhatia SN, Hao L. Journal: Journal of visualized experiments : JoVE - CRISPR-Cas9 genome editing provides a means for simple and scalable production of gene knockouts in mammalian cell lines. The development of guide RNA (gRNA) libraries targeting tens of thousands of genes has allowed researchers to produce pools of
Date: (2022) Authors: Graham MacLeod , Nishani Rajakulendran , Stephane Angers Ref: Cancer Drug Resistance - Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9. new window
Date: 2023-12-08 Authors: Zhu Y, Tahara SM, Tsukamoto H, Machida K. Journal: STAR protocols - CRISPR-Cas9-based screening technologies enable precise, high-throughput genetic and epigenetic manipulation to study mechanisms of development and disease and identify new therapeutic targets. Here, we describe a general protocol for the generation
Date: (2022) Authors: Sophia DeLuca , Nenad Bursac Ref: Cardiac Tissue Engineering - Protocol for replacing coding intronic MiMIC and CRIMIC lines with T2A-split-GAL4 lines in Drosophila using genetic cros... new window
Date: 2023-12-06 Authors: Li SA, Li HG, Shoji N, Desplan C, Chen YD. Journal: STAR protocols - The CRISPR/Cas9 system, a defense mechanism naturally occurring in prokaryotes, has been recently repurposed as an RNA-guided DNA targeting platform and widely used as a powerful tool for genome editing. Here we describe how to modify the
Date: (2022) Authors: Alessia Ametrano , Maria Rosaria Coscia Ref: Marine Genomics Date: 2023-12-03 Authors: Yang X, Duan S, Li Z, Wang Z, Kon N, Zhang Z, Gu W. Journal: STAR protocols