Results 681-690 of about 1,000
- Targeting the AAVS1 Site by CRISPR/Cas9 with an Inducible Transgene Cassette for the Neuronal Differentiation of Human P... new windowCRISPR/Cas9 system is a powerful genome-editing technology for studying genetics and cell biology. Safe harbor sites are ideal genomic locations for transgene integration with minimal interference in cellular functions. Gene targeting of the AAVS1
Date: (2022) Authors: Jinchao Gu , Ben Rollo , Huseyin Sumer , Brett Cromer Ref: Applications of Genome Modulation and Editing - Protocol for generating mutant zebrafish using CRISPR-Cas9 followed by quantitative evaluation of vascular formation. new window
Date: 2023-12-02 Authors: Luo J, Lu C, Wang M, Yang X. Journal: STAR protocols - CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy new windowThe discovery of CRISPR/Cas9 as an easily programmable endonuclease heralds a new era of genetic manipulation. With this comes the prospect of novel gene therapy approaches, and the potential to cure previously untreatable genetic diseases. However,
Date: (2022) Authors: Matthew D. Newton , Benjamin J. Taylor , Maria Emanuela Cuomo , David S. Rueda Ref: Optical Tweezers - Protocol for CRISPR-Cas9-mediated genome editing to study spermatogenesis in Caenorhabditis elegans new window
Date: 2023-11-17 Authors: Wang P, Cao Z, Wang Q, Ma X, Wang N, Chen L, Zhao Y, Miao L. Journal: STAR protocols - CRISPR-Cas9 is a convenient tool to create knockdown mutants for desired genes. Early generations of transgenic plants usually have multiple editing events. To select the best plants for future study, these plants need to be screened for editing type
Date: (2022) Authors: Junli Zhang Ref: Genomics of Cereal Crops - Gene editing introduces stable mutations into the genome and has powerful applications extending from research to clinical gene therapy. CRISPR-Cas9 gene editing can be employed to study directly the functional impact of stable gene knockout,
Date: (2022) Authors: Katherine Shortt , Daniel P. Heruth Ref: Hepatocytes Date: 2023-11-09 Authors: Sahu S, Sullivan T, Southon E, Caylor D, Geh J, Sharan S. Journal: STAR protocols - Reprogramming Mouse Oviduct Epithelial Cells Using In Vivo Electroporation and CRISPR/Cas9-Mediated Genetic Manipulation new windowAdvances in gene editing tools such as CRISPR/Cas9 have made precise in vivo gene editing possible, opening up avenues of research into somatic cell reprograming to study adult stem cells, homeostasis, and malignant transformation. Here we describe a
Date: (2022) Authors: Matthew J. Ford , Yojiro Yamanaka Ref: Stem Cell Assays - DNA methylation is an epigenetic modification with an established role in both normal cellular function and mammalian disease. Despite well-characterized associations between aberrant DNA methylation changes and gene expression, evidence for a causal
Date: (2022) Authors: Jim Smith , Rakesh Banerjee , Robert J. Weeks , Aniruddha Chatterjee Ref: Chromatin Date: 2023-11-06 Authors: Papaioannou VE, Behringer RR. Journal: Cold Spring Harbor protocols