Results 761-770 of about 1,000
- Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays. new window
Date: 2023-07-31 Authors: Tse SY, Pukkila-Worley R. Journal: STAR protocols - The CRISPR revolution has enabled greater manipulation of many genomes to help elucidate gene function and drive biological gains. This chapter will describe in detail the steps required to target and edit either a single gene or multiple loci in the
Date: (2022) Authors: Matthew J. Milner , Emma J. Wallington Ref: Accelerated Breeding of Cereal Crops - CRISPR generation of CSF1R-G795A human microglia for robust microglia replacement in a chimeric mouse model. new window
Date: 2023-07-29 Authors: Chadarevian JP, Davtyan H, Lombroso SI, Bennett FC, Blurton-Jones M. Journal: STAR protocols - Over the last decade, the use of auxin-inducible degrons (AID) to control the stability of target proteins has revolutionized the field of cell biology. AID-mediated degradation helps to overcome multiple hurdles that have been encountered in
Date: (2022) Authors: Vasilisa Aksenova , Alexei Arnaoutov , Mary Dasso Ref: The Nuclear Pore Complex Date: 2023-07-22 Authors: Ludwik KA, Telugu N, Schommer S, Stachelscheid H, Diecke S. Journal: STAR protocols - Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology new windowActive, hands-on learning has been shown to improve educational outcomes in STEM subjects. However, implementation of hands-on activities for teaching biology has lagged behind other science disciplines due to challenges associated with the use of
Date: (2022) Authors: Ally Huang , Bruce Bryan , Sebastian Kraves , Ezequiel Alvarez-Saavedra , Jessica C. Stark Ref: Cell-Free Gene Expression - Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR. new window
Date: 2023-07-09 Authors: Wu L, Tan JC, Gascoigne NRJ. Journal: STAR protocols - Discovery of the genetic components underpinning fundamental and disease-related processes is being rapidly accelerated by combining efficient, programmable genetic engineering with phenotypic readouts of high spatial, temporal and/or molecular
Date: (2022) Authors: David Feldman , Luke Funk , Anna Le , Rebecca J. Carlson ... Paul C. Blainey - Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies. new window
Date: 2023-07-09 Authors: du Halgouet A, Darbois A, Alphonse A, Yvorra T, Colombeau L, Rodriguez R, Lantz O, Salou M. Journal: STAR protocols - Editing the Drosophila genome is incredibly useful for gene functional analysis. However, compared to gene knockouts, precise gene editing is difficult to achieve. Prime editing, a recently described CRISPR/Cas9-based technique, has the potential to
Date: (2022) Authors: Justin A. Bosch , Norbert Perrimon Ref: Drosophila