Results 781-790 of about 1,000
- Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System. new window
Date: 2023-06-23 Authors: Xu MF, Chen J, Xu Y, Zhang JL, Zhou Y, He JJ, Wu S, Wei YL, She ZY. Journal: Journal of visualized experiments : JoVE - Neural circuit activity representing sensorimotor experiences trigger molecular mechanisms that drive long-lasting changes in brain circuits underlying learning and memory. Recent advancements in molecular genetics have led to development of rich
Date: (2022) Authors: Pamela Valnegri , Tomoko Yamada , Yue Yang Ref: Measuring Cerebellar Function - Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatur... new window
Date: 2023-06-23 Authors: Grausam KB, Breunig JJ. Journal: Journal of visualized experiments : JoVE - Creating mouse models of human genetic disease (Gurumurthy and Lloyd, Dis Models Mech 12(1):dmm029462, 2019) and livestock trait (Schering et al. Arch Physiol Biochem 121(5):194–205, 2015; Habiela et al. J Gen Virol 95 (Pt 11):2329–2345, 2014) have
Date: (2022) Authors: Jun Liu , Paul J. Verma Ref: Applications of Genome Modulation and Editing Date: 2023-06-23 Authors: Egger T, Aze A, Maiorano D. Journal: STAR protocols - Genome engineering technologies enable targeted mutations to be induced at almost any location in plant genomes. In particular, Cas9 nucleases use easily recoded RNA guides to target user-defined sequences and generate double-stranded breaks (DSB)
Date: (2022) Authors: Quentin M. Dudley , Oleg Raitskin , Nicola J. Patron Ref: Plant Synthetic Biology Date: 2023-06-16 Authors: Takahashi G, Miyaoka Y. Journal: STAR protocols - Single-domain antibodies such as nanobodies (Nbs) have substantially expanded the possibilities of advanced cellular imaging. In comparison to conventional antibodies, Nbs are characterized by small size, high stability, and solubility in many
Date: (2022) Authors: Funmilayo O. Fagbadebo , Ulrich Rothbauer Ref: Single-Domain Antibodies - Protocol for quantitative analysis of RNA 3'-end processing induced by disassociated subunits using chromatin-associated... new window
Date: 2023-06-15 Authors: Huang J, Bao L, Zhu J, Ji X. Journal: STAR protocols - dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy new windowMany disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate
Date: (2022) Authors: Lisa M. Riedmayr , Klara S. Hinrichsmeyer , Nina Karguth , Sybille Böhm ... Elvir Becirovic