Results 121-130 of about 1,000
- Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid subs... new windowRobust expression of guide RNA (gRNA) is essential for successful implementation of CRISPR–Cas9 genome-editing methods. The gRNA components, such as an RNA polymerase promoter followed by the gRNA coding sequence and an RNA polymerase terminator
Date: (2025) Authors: Roman Teo Oliynyk , George M. Church - Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and p... new window
Date: 2025-02-07 Authors: Nityanandam A, Patton MH, Bayazitov IT, Newman KD, Thomas KT, Zakharenko SS. Journal: STAR protocols Authors: "Domenico Modaffari","Edward Wallace","Kenneth Sawin", Authors: "Luke Bradley", - Genome-wide screens are a powerful technique to dissect the complex network of genes regulating diverse cellular phenotypes. The recent adaptation of the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here,
Date: (2025) Authors: Arnold Bolomsky , Jaewoo Choi , James D. Phelan Ref: Lymphoma Date: 2025-02-05 Authors: Osgood NRB, Zawalick NM, Sawyer CB, Cowan QT, Gu S, Mawson SJ, Ranzau BL, Li L, Gymrek M, Goren A, Komor AC. Journal: Methods in enzymology Authors: "David Booth", - CRISPR-Mediated Construction of Gene-Knockout Mice for Investigating Antiviral Innate Immunity new windowWith the rapid development of CRISPR-Cas9 technology, gene editing has become a powerful tool for studying gene function. Specifically, in the study of the mechanisms by which natural immune responses combat viral infections, gene knockout mouse
Date: (2025) Authors: Yangkun Shen , Xiangqian Zhao , Chunfu Zheng , Qi Chen Ref: Antiviral Innate Immunity Date: 2025-02-03 Authors: Elfimov KA, Baboshko DA, Gashnikova NM. Journal: Methods and protocols Authors: "Yun Li",