Results 311-320 of about 1,000
- Correlated Single-Molecule Magnetic Tweezers and Fluorescence Measurements of DNA-Enzyme Interactions new windowCombining force spectroscopy and fluorescence microscopy provides a substantial improvement to the single-molecule toolbox by allowing simultaneous manipulation and orthogonal characterizations of the conformations, interactions, and activity of
Date: (2024) Authors: Julene Madariaga-Marcos , Pierre Aldag , Dominik J. Kauert , Ralf Seidel Ref: Single Molecule Analysis Date: 2024-12-04 Authors: Ai X, Tang Z. Journal: Methods in enzymology - The ENCODE Consortium’s efforts to annotate noncoding cis-regulatory elements (CREs) have advanced our understanding of gene regulatory landscapes. Pooled, noncoding CRISPR screens offer a systematic approach to investigate cis-regulatory mechanisms.
Date: (2024) Authors: David Yao , Josh Tycko , Jin Woo Oh , Lexi R. Bounds ... Steven K. Reilly Date: 2024-11-29 Authors: Kolodziejczyk A, Hoffmann J, Cubillos P, Albert M. Journal: Journal of visualized experiments : JoVE - Introduction or knock-in of precise genomic modifications remains one of the most important applications of CRISPR/Cas9 in all model systems including zebrafish. The most widely used type of donor template containing the desired modification is
Date: (2024) Authors: Sergey V. Prykhozhij , Jason N. Berman Ref: Zebrafish - Protocol for detecting and quantifying hATF4-HA in non-stress versus stress conditions using automated and quantitative ... new window
Date: 2024-11-20 Authors: Smirnova AM, Herrmannová A, Valášek LS. Journal: STAR protocols - Gene Editing and Reprogramming of Human Fibroblast Cells to Human Induced Pluripotent Stem Cells new windowPredictive disease models play significant roles in advancing our knowledge of the pathology of human disease. In this field, animal models have been extensively employed and have provided crucial insights into the pathophysiological mechanisms of
Date: (2024) Authors: Zhe Zhang , Shinghua Ding Ref: Human Induced Pluripotent Stem Cells - New vectors and optimal conditions for allelic exchange in hypervirulent Klebsiella pneumoniae. new window
Date: 2024-11-19 Authors: Van Allen ME, Chandrashekarappa DG, Bina XR, Bina JE. Journal: Journal of microbiological methods - Recent advances in patient-derived induced Pluripotent Stem Cell (iPSC) generation, improvement of cardiomyocyte-directed differentiation protocols, and the availability of new genome editing techniques have opened up new avenues for disease modeling
Date: (2024) Authors: Christopher Cavanaugh , Jennifer Hesson , Julie Mathieu Ref: Familial Cardiomyopathies - Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro. new window
Date: 2024-11-15 Authors: Cui Z, Yuan P, Zhao Z, Zhao F, Lu L. Journal: Journal of visualized experiments : JoVE