Results 351-360 of about 1,000
- Protocol for the efficient and inducible generation of CRISPR-Cas9-edited human cortical neurons from the iCas9-iNgn2 hP... new window
Date: 2024-09-28 Authors: Dhaliwal NK, Weng OY, Li Y. Journal: STAR protocols - The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID)
Date: (2024) Authors: Tara Hicks , Nicola Silva , Sarit Smolikove Ref: Meiosis - Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening. new window
Date: 2024-09-27 Authors: Harrell RA. Journal: Journal of visualized experiments : JoVE - The advent of locus-specific protein recruitment technologies has enabled a new class of studies in chromatin biology. Epigenome editors (EEs) enable biochemical modifications of chromatin at almost any specific endogenous locus. Their
Date: (2024) Authors: Z. Begum Yagci , Gautami R. Kelkar , Tyler J. Johnson , Dilara Sen , Albert J. Keung Ref: Epigenome Editing - Protocol for the neonatal intracerebroventricular delivery of adeno-associated viral vectors for brain restoration of ME... new window
Date: 2024-09-25 Authors: Yang K, Li T, Geng Y, Zhang R, Xu Z, Wu J, Yuan Y, Zhang Y, Qiu Z, Li F. Journal: STAR protocols - ChEC-Seq: A Comprehensive Guide for Scalable and Cost-Efficient Genome-Wide Profiling in Saccharomyces cerevisiae new windowChromatin endogenous cleavage coupled with high-throughput sequencing (ChEC-seq) is a profiling method for protein-DNA interactions that can detect binding locations in vivo, does not require antibodies or fixation, and provides genome-wide coverage
Date: (2024) Authors: Tamar Gera , Divya Krishna Kumar , Gilad Yaakov , Naama Barkai , Felix Jonas Ref: Chromatin Immunoprecipitation - Recent Advances in Agrobacterium -Mediated Maize Genetic Transformation Using Immature Embryos and Seedling Leaf W... new window
Date: 2024-09-19 Authors: Azanu MK, Kang M, Lee K, Wang K. Journal: Cold Spring Harbor protocols - Evaluating Chromosome Instability and Genotoxicity Through Single Cell Quantitative Imaging Microscopy new windowAcross eukaryotes, genome stability is essential for normal cell function, physiology, and species survival. Aberrant expression of key genes or exposure to genotoxic agents can have detrimental effects on genome stability and contribute to the
Date: (2024) Authors: Rubi Campos Gudiño , Kailee A. Rutherford , Kirk J. McManus Ref: Cancer Cytogenetics and Cytogenomics - Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector. new window
Date: 2024-09-18 Authors: Yen KK, Terlecky AJ, Hao M, Cienfuegos V, Rojtman A, Chen L, Kreiswirth BN. Journal: Journal of microbiological methods - Recording molecular information to genomic DNA is a powerful means of investigating topics ranging from multicellular development to cancer evolution. With molecular recording based on genome editing, events such as cell divisions and signaling
Date: (2024) Authors: Hanna Liao , Junhong Choi , Jay Shendure