Results 411-420 of about 1,000
- Cultivated potato (Solanum tuberosum L.) is one of the most important staple food crops worldwide. Its tetraploid and highly heterozygous nature poses a great challenge to its basic research and trait improvement through traditional mutagenesis
Date: (2023) Authors: Matías Nicolás González , Gabriela Alejandra Massa , Mariette Andersson , Leonardo Storani ... Sergio Enrique Feingold Ref: Plant Genome Engineering - Protocol for generating in-frame seamless knockins in Drosophila using the SEED/Harvest technology. new window
Date: 2024-07-11 Authors: Aguilar G, Bauer M, Vigano MA, Guerrero I, Affolter M. Journal: STAR protocols - Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze
Date: (2023) Authors: Konstantinos Sousounis , Katharine Courtemanche , Jessica L. Whited Ref: Salamanders - Protocol for establishing knockout cell clones by deletion of a large gene fragment using CRISPR-Cas9 with multiple guid... new window
Date: 2024-07-06 Authors: Saito AC, Higashi T, Chiba H. Journal: STAR protocols - Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) (CRISPR-Cas9) gene editing enables rapid production of genome-edited animals. The Cas9/guide RNA (gRNA) component can be introduced into zygotes in
Date: (2023) Authors: Takuro Horii , Izuho Hatada Ref: Genome Editing in Animals Date: 2024-07-01 Authors: Harrell RA. Journal: Cold Spring Harbor protocols - A specific targeting nuclease is a powerful tool for mediating genome alternative expression with high precision. The RNA sequence-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive
Date: (2023) Authors: Ya-Ju Chang , Joseph Ryu , Xuan Cui , Stephen H. Tsang Ref: Retinitis Pigmentosa - Protocol for linking enhanced interferon immunity to virus resistance in gene-knockout zebrafish. new window
Date: 2024-06-26 Authors: Qu ZL, Zhang YB. Journal: STAR protocols - Prime Editing for the Installation and Correction of Mutations Causing Inherited Retinal Disease: A Brief Methodology new windowInherited retinal diseases (IRDsInherited retinal dystrophies/Inherited retinal diseases (IRDs)) encompass a large heterogeneous group of rare blinding disorders whose etiology originates from mutations in the 280 genes identified to date. Clustered
Date: (2023) Authors: Yi-Ting Tsai , Bruna Lopes Costa , Nicholas D. Nolan , Salvatore Marco Caruso ... Peter M. Quinn Ref: Retinitis Pigmentosa - Protocol for genomic editing in human resting primary NK cells and NK-92 cells via CRISPR-Cas9 ribonucleoproteins. new window
Date: 2024-06-26 Authors: Verhezen T, Lau HW, Van Audenaerde J, Wouters A, Smits E, De Waele J. Journal: STAR protocols