Results 461-470 of about 1,000
Date: 2024-04-09 Authors: Lankford KP, Hulleman JD. Journal: STAR protocols - CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding new windowCRISPR–Cas nuclease systems, base editors, and CRISPR activation have greatly advanced plant genome engineering. However, the combinatorial approaches for multiplexed orthogonal genome editing and transcriptional regulation were previously
Date: (2023) Authors: Changtian Pan , Yiping Qi Date: 2024-03-27 Authors: Akter S, Kamal E, Schwarz C, Lewin A. Journal: Journal of microbiological methods - Fluorescence in situ hybridization (FISH) has been widely used to visualize target DNA sequences in fixed chromosome samples by denaturing the dsDNA to allow complementary probe hybridization, thus damaging the chromatin structure by harsh
Date: (2023) Authors: Bhanu Prakash Potlapalli , Takayoshi Ishii , Kiyotaka Nagaki , Saravanakumar Somasundaram , Andreas Houben Ref: Plant Cytogenetics and Cytogenomics - CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation. new window
Date: 2024-03-15 Authors: J Davis D, McNew JF, Walls JN, Bethune CE, Oswalt PS, Bryda EC. Journal: Journal of visualized experiments : JoVE - CRISPR/Cas9 as a Simple Technique for the Generation of Murine Knockout Models for Neuropsychiatric Diseases new windowThe development of schizophrenia-like rodent models is still a major challenge for the study of this mental disorder. Schizophrenia and other neuropsychiatric disorders are thought to be triggered by multiple factors, and furthermore, the genetic
Date: (2023) Authors: Lide Totorikaguena , Estibaliz Olabarrieta Ref: Schizophrenia - Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes. new window
Date: 2024-03-08 Authors: Biase FH, Schettini G. Journal: STAR protocols - Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout Using CRISPR-BETS new windowCytosine base editors (CBEs) accurately modify target sites by mediating a C to T change (or a G to A change on the opposite strand). This allows us to install premature stop codons for gene knockout. However, highly specific sgRNAs (single-guide
Date: (2023) Authors: Yuechao Wu , Tao Zhang Ref: Plant Genome Engineering - Generation of immunocompetent somatic glioblastoma mouse models through in situ transformation of subventricular zone ne... new window
Date: 2024-03-01 Authors: Clements M, Simpson Ragdale H, Garcia-Diaz C, Parrinello S. Journal: STAR protocols - Genetic editing of hematopoietic stem and progenitor cells can be employed to understand gene-function relationships underlying hematopoietic cell biology, leading to new therapeutic approaches to treat disease. The ability to collect, purify, and
Date: (2023) Authors: Reza Shahbazi , Patricia Lipson , Karthikeya S. Gottimukkala , Daniel D. Lane , Jennifer E. Adair Ref: Hematopoietic Stem Cells