Results 481-490 of about 1,000
- Rapid detection of infectious and zoonotic diseases is very important for pathogen identification and infection control. Molecular diagnostic assays are well-known for high accuracy and sensitivity; however, conventional methods such as real-time PCR
Date: (2023) Authors: Linxian Li , Shiyuan Li , Dayong Gu , Yong Xu , Jin Wang Ref: Clinical Applications of Nucleic Acid Amplification - Optimizing Visualization of Axonal Transport of Endogenous Cargo by Fluorescence Microscopy in Living Caenorhabditis ele... new window
Date: 2024-02-16 Authors: Glomb O, Lyu M, Yogev S. Journal: Journal of visualized experiments : JoVE - In recent years CRISPR-Cas9 knockouts (KO) have become increasingly utilized to study gene function. MicroRNAs (miRNAs) are short noncoding RNAs, 20–25 nucleotides long, which affect gene expression through posttranscriptional repression. As miRNAs
Date: (2023) Authors: Alice M. Godden , Marco Antonaci , Grant N. Wheeler Ref: MicroRNA Detection and Target Identification - Multiplexed CRISPR-Cas9 protocol for large transgene integration into the Schistosoma mansoni genome. new window
Date: 2024-02-13 Authors: Ittiprasert W, Moescheid MM, Mann VH, Brindley PJ. Journal: STAR protocols - The mosquito-borne West Nile virus (WNV) poses a great threat to public health as no vaccine or specific antiviral treatment is available. Exploring virus-host interactions, specifically host factors that are required for virus infection, is
Date: (2023) Authors: Rong Zhang Ref: West Nile Virus - The development of RT-RPA and CRISPR-Cas12a based assay for sensitive detection of infectious hematopoietic necrosis vir... new window
Date: 2024-02-07 Authors: Rong F, Wang H, Tang X, Xing J, Sheng X, Chi H, Zhan W. Journal: Journal of virological methods - Large-scale genetic screens are becoming increasingly used as powerful tools to query the genome to identify therapeutic targets in cancer. The advent of the CRISPR technology has revolutionized the effectiveness of these screens and has made it
Date: (2023) Authors: Vincent Maranda , Yue Zhang , Frederick S. Vizeacoumar , Andrew Freywald , Franco J. Vizeacoumar Ref: The Tumor Microenvironment Date: 2024-01-31 Authors: Yu F, Liu S, Zhu AC, He C, Qian Z. Journal: STAR protocols - Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades
Date: (2023) Authors: Katharina Kohm , Veronika Theresa Lutz , Ines Friedrich , Robert Hertel Ref: Metagenomics - Protocol for the quantitative identification of endogenously ISGylated proteins from mammalian cell lines. new window
Date: 2024-01-29 Authors: Wardlaw CP, Miele MM, Li Z, Hendrickson RC, Petrini JHJ. Journal: STAR protocols