Results 591-600 of about 1,000
Date: 2024-01-01 Authors: Pérez-García I, Pérez-García V. Journal: Methods in molecular biology (Clifton, N.J.) - Maintenance and self-renewal of the spermatogonial stem cell (SSC) population in the testis are dictated by the expression of a unique suite of genes. In manipulating gene expression through loss-of-function approaches, we can identify important
Date: (2023) Authors: Connor Cason , Tessa Lord Ref: Spermatogonial Stem Cells - Functional Interrogation of Ca2+ Signals in Human Cancer Cells In Vitro and Ex Vivo by Fluorescent Microscopy and Molecu... new windowGenetically encoded calcium indicators (GECIs) and high-resolution confocal microscopy enable dynamic visualization of calcium signals in cells and tissues. Two-dimensional and 3D biocompatible materials mimic the mechanical microenvironments of
Date: (2023) Authors: Chenyu Liang , Miao Huang , Mai Tanaka , Suzanne Lightsey ... Xin Tang Ref: Microfluidic Systems for Cancer Diagnosis Date: 2024-01-01 Authors: Shaikh MV, Custers S, Anand A, Miletic P, Venugopal C, Singh SK. Journal: Methods in molecular biology (Clifton, N.J.) - As a miniature RNA-guided endonuclease, IscB is presumed to be the ancestor of Cas9 and to share similar functions. IscB is less than half the size of Cas9 and thus more suitable for in vivo delivery. However, the poor editing efficiency of IscB in
Date: (2023) Authors: Dingyi Han , Qingquan Xiao , Yifan Wang , Hainan Zhang ... Yingsi Zhou - Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing new windowExtracellular matrix (ECM) rigidity serves as a crucial mechanical cue impacting diverse biological processes. However, understanding the molecular mechanisms of rigidity sensing has been limited by the spatial resolution and force sensitivity of
Date: (2023) Authors: Wenxu Wang , Wei Chen , Chaoyang Wu , Chen Zhang ... Zheng Liu - Metabolite-Based Genome-Wide Association Studies of Large-Scale Metabolome Analysis to Illustrate Alterations in the Met... new windowGiven that anthropogenic activities are evoking a profound effect on the climate resulting in more extreme events such as severe drought and heat waves while global demand for food is ever-increasing, understanding plant responses to stresses is
Date: (2023) Authors: Feng Zhu , Mustafa Bulut , Yunjiang Cheng , Saleh Alseekh , Alisdair R. Fernie Ref: Plant Abiotic Stress Signaling - Oligonucleotide ligands (DNA, RNA, or XNA), also known as aptamers, are selected against various target molecules using an iterative, evolutionary process called systematic evolution of ligands by exponential enrichment (SELEX). To select aptamers
Date: (2023) Authors: Nicole Williams , Rutika Patel , Prabodhika Mallikaratchy Ref: Nucleic Acid Aptamers - Sirtuins are identified as NAD+-dependent class III histone deacetylases (HDAC) and are involved in a variety of cellular activities, including energy metabolism, DNA repair, epigenetics, gene expression, cell proliferation, differentiation, and
Date: (2023) Authors: Shimin Sun , Xiaojiao Xia , Ming Wang , Baohua Liu Ref: HDAC/HAT Function Assessment and Inhibitor Development Date: 2024-01-01 Authors: Gao J, Cheng J, Lian J. Journal: Methods in molecular biology (Clifton, N.J.)