Results 691-700 of about 1,000
- Characterization of Mammalian In Vivo Enhancers Using Mouse Transgenesis and CRISPR Genome Editing new windowEmbryonic morphogenesis is strictly dependent on tight spatiotemporal control of developmental gene expression, which is typically achieved through the concerted activity of multiple enhancers driving cell type-specific expression of a target gene.
Date: (2022) Authors: Marco Osterwalder , Stella Tran , Riana D. Hunter , Eman M. Meky ... Axel Visel Ref: Craniofacial Development Date: 2023-11-06 Authors: Papaioannou VE, Behringer RR. Journal: Cold Spring Harbor protocols - The CRISPR/Cas9 is being developed as an invaluable system that allows rapid and site-specific genome editing in a wide variety of organisms, including diverse insects. It has been successfully used for gene function annotations of RNAi pathway in
Date: (2022) Authors: Rihui Yan , Xianwu Lin Ref: RNAi Strategies for Pest Management Date: 2023-11-06 Authors: Papaioannou VE, Behringer RR. Journal: Cold Spring Harbor protocols - A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9 new windowHuman pluripotent stem cells (hPSCs) have the capacity for self-renewal and differentiation into most cell types and, in contrast to widely used cell lines, are karyotypically normal and non-transformed. Hence, hPSCs are considered the gold-standard
Date: (2022) Authors: Barbara Mair , Michael Aregger , Amy H. Tong , Katherine S. Chan , Jason Moffat Ref: Essential Genes and Genomes - Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles. new window
Date: 2023-11-03 Authors: Richardson TM, Pettway YD, Walker JT, Nelson HA, Ishahak M, Poffenberger G, Aramandla R, Reihsmann C, Agarwal A, Powers AC, Brissova M. Journal: Journal of visualized experiments : JoVE - A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins new windowDNA double-strand breaks (DSBs) are mainly repaired by homologous recombination (HR) and non-homologous end joining (NHEJ). The choice of HR or NHEJ is dictated in part by whether the broken DNA ends are resected to generate extended single-stranded
Date: (2022) Authors: Bo-Ruei Chen , Barry P. Sleckman Ref: DNA Damage Responses - Protocol for arrayed gRNA screening by base editors in mammalian cell lines using lentiviral system. new window
Date: 2023-11-03 Authors: Ravi NS, George A, Mohankumar KM. Journal: STAR protocols - Targeted Gene Disruption Via CRISPR/Cas9 Ribonucleoprotein Complexes in Fusarium oxysporum new windowCharacterization of a gene of interest frequently relies on generation of a mutant as a critical component. Transformation to disrupt a gene has been previously accomplished by several methods in Fusarium oxysporum. Here we provide a detailed method
Date: (2022) Authors: Ambika Pokhrel , Seungyeon Seo , Qiang Wang , Jeffrey J. Coleman Ref: Fusarium wilt - Detection of the DNA binding of transcription factors in situ at the single-cell resolution in cultured cells by proximi... new window
Date: 2023-11-02 Authors: Ram BM, Dai C. Journal: STAR protocols