Results 711-720 of about 1,000
  1. Harnessing CRISPR-Cas9 for Epigenetic Engineering new window
    Epigenome editing has become more precise and effective by coupling epigenetic effectors to the dCas9 protein and targeting regulatory regions such as promoters and enhancers. Here, we describe a basic methodology for performing an epigenome editing
    Date: (2022)
    Authors: Rosa S. Guerra-Resendez , Isaac B. Hilton
    Ref: Riboregulator Design and Analysis
    https://experiments.springernature.com/.../978-1-0716-2421-0_14 - SpringerNature
  2. Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm. new window
    Date: 2023-10-13
    Authors: Cohen R, Sosnicki DM, White MA, Nelson JL, Mukai C, Travis AJ.
    Journal: Journal of visualized experiments : JoVE
    https://doi.org/10.3791/65962 - Europe PMC
  3. Spatial CRISPR screens in tumors new window
    Perturb-map combines multiplex imaging and spatial transcriptomics to dissect complex biological functions from CRISPR screens in tumor tissues.
    Date: (2022)
    Authors: Lei Tang
    https://www.nature.com/articles/s41592-022-01501-7 - SpringerNature
  4. Generating Retinal Injury Models in Xenopus Tadpoles. new window
    Date: 2023-10-13
    Authors: Parain K, Donval A, Chesneau A, Lun JX, Borday C, Perron M.
    Journal: Journal of visualized experiments : JoVE
    https://doi.org/10.3791/65771 - Europe PMC
  5. Tracing and Manipulating Drosophila Cell Lineages Based on CRISPR: CaSSA and CLADES new window
    Cell lineage defines the mitotic connection between cells that make up an organism. Mapping these connections in relation to cell identity offers an extraordinary insight into the mechanisms underlying normal and pathological development. The
    Date: (2022)
    Authors: Jorge Garcia-Marques , Tzumin Lee
    Ref: Drosophila
    https://experiments.springernature.com/.../978-1-0716-2541-5_9 - SpringerNature
  6. CAR1 as a new selective marker for genetic engineering of wine yeasts. new window
    Date: 2023-10-10
    Authors: Urakov VN, Mardanov AV, Alexandrov AI, Ruzhitskiy AO, Ravin NV, Kushnirov VV.
    Journal: Journal of microbiological methods
    https://doi.org/10.1016/j.mimet.2023.106840 - Europe PMC
  7. Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster new window
    The Notch (N) signaling pathway plays versatile roles in development and disease of many model organisms (Andersson et al., Development 138, 3593–3612, 2011; Hori et al., J Cell Sci, 126, 2135–2140, 2013). However, studying a role for N in adult
    Date: (2022)
    Authors: Cordula Schulz
    Ref: Notch Signaling Research
    https://experiments.springernature.com/.../978-1-0716-2201-8_13 - SpringerNature
  8. Protocol for selecting single human pluripotent stem cells using a modified micropipetter. new window
    Date: 2023-10-09
    Authors: Mojica-Perez SP, Stokes K, Jaklic DC, Jahagirdar S, Uhler M, Parent JM, Niu W.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2023.102629 - Europe PMC
  9. A Method to Reduce off-Targets in CRISPR/Cas9 System in Plants new window
    One of the strategies to reduce the off-target mutations in CRISPR/Cas9 system is to use the temperature-independent gene transformation method. Mesoporous silica nanoparticles (MSNs)-gene delivery system is temperature-independent; thus, it can
    Date: (2022)
    Authors: Ali Movahedi , Zahra Hajiahmadi , Hui Wei , Liming Yang ... Qiang Zhuge
    Ref: Plant Gene Silencing
    https://experiments.springernature.com/.../978-1-0716-1875-2_21 - SpringerNature
  10. High-Throughput Mutant Screening via Transposon Sequencing. new window
    Date: 2023-10-03
    Authors: Bourgeois J, Camilli A.
    Journal: Cold Spring Harbor protocols
    https://doi.org/10.1101/pdb.top107867 - Europe PMC
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