Results 721-730 of about 1,000
- A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Ve... new windowCells with a loss-of-function mutation in a gene (knockout cells) are powerful tools for characterizing the function of such gene product. However, for essential genes, conditional knockout cell lines must be generated. The auxin-inducible degron
Date: (2022) Authors: Kohei Nishimura , Tatsuo Fukagawa Ref: Essential Genes and Genomes - Identifying essential long non-coding RNAs in cancer using CRISPRi-based dropout screens. new window
Date: 2023-09-28 Authors: Bril O, Schwarzmueller LJ, Moreno LF, Vermeulen L, Léveillé N. Journal: STAR protocols - Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing new windowVesicle trafficking entails packaging and transport of membrane-associated proteins to their target membranes, and recycling or degradation of endocytosed proteins. Biochemical and cell biological studies of vesicle trafficking often require the
Date: (2022) Authors: Syed Mukhtar Ahmed Ref: Membrane Trafficking Date: 2023-09-27 Authors: Zhou B, Chen Y, Li L, Liu J, Wang Y, Huang Z, Hu Z, Tian R, Li Z. Journal: STAR protocols - CLOuD9: CRISPR-Cas9-Mediated Technique for Reversible Manipulation of Chromatin Architecture new windowThe spatial organization of the genome plays a critical role in cell-specific biological functions such as gene expression. Existing genome-wide technologies reveal a dynamic interplay between chromatin looping and gene regulation, but the mechanisms
Date: (2022) Authors: Wei Qiang Seow , Poonam Agarwal , Kevin C. Wang Ref: Spatial Genome Organization - Optogenetic induction of alpha-synuclein aggregation in human dopaminergic neurons to model Parkinson's disease patholog... new window
Date: 2023-09-23 Authors: Ra EA, Kim MS, Lee G. Journal: STAR protocols - The bacterial genus Lactobacillus comprises a vast range of strains with varying metabolic and probiotic traits, with genome editing representing an essential tool to probe genotype–phenotype relationships and enhance their beneficial properties.
Date: (2022) Authors: Justin M. Vento , Chase L. Beisel Ref: Recombineering - Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells. new window
Date: 2023-09-21 Authors: Wu Y, Sidharta M, Zhong A, Persily B, Li M, Zhou T. Journal: STAR protocols - Ligand Identification for Orphan MHC-Agnostic T-Cell Receptors by Whole Genome CRISPR–Cas9 Screening new windowKiller T-cells play important roles in immunity to infection and cancer by detecting intracellular anomalies at the cell surface and destroying the cells that bear them. Conventional killer T-cells scan the intracellular proteome by sampling peptides
Date: (2022) Authors: Michael D. Crowther , Mateusz Legut , Andrew K. Sewell Ref: T-Cell Repertoire Characterization Date: 2023-09-21 Authors: Wu X, Han J. Journal: STAR protocols