Results 731-740 of about 1,000
- The history of DNA manipulation for the creation of genetically modified animals began in the 1970s, using viruses as the first DNA molecules microinjected into mouse embryos at different preimplantation stages. Subsequently, simple DNA plasmids were
Date: (2022) Authors: Lluis Montoliu Ref: Applications of Genome Modulation and Editing - Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity l... new window
Date: 2023-09-20 Authors: Shi L, Marti Ferrando T, Landeo Villanueva S, Joosten MHAJ, Vleeshouwers VGAA, Bachem CWB. Journal: STAR protocols - Ribonucleoproteins (RNPs) are RNA-protein complexes utilized natively in both prokaryotes and eukaryotes to regulate essential processes within the cell. Over the past few years, many of these native systems have been adapted to provide control over
Date: (2022) Authors: Trevor R. Simmons , Andrew D. Ellington , Lydia M. Contreras Ref: Riboregulator Design and Analysis - Generation of mouse models carrying B cell restricted single or multiplexed loss-of-function mutations through CRISPR-Ca... new window
Date: 2023-09-18 Authors: Ten Hacken E, Gruber M, Hernández-Sánchez M, Hoffmann GB, Baranowski K, Redd RA, Clement K, Livak K, Wu CJ. Journal: STAR protocols - The CRISPR/Cas technology has recently become the tool of choice for gene editing applications in plants. Here we describe the method of assembling CRISPR/Cas DNA constructs, using the modular cloning (MoClo), for deployment in cereal species. For
Date: (2022) Authors: Florian Hahn , Laura Sanjurjo Loures , Vladimir Nekrasov Ref: Accelerated Breeding of Cereal Crops - Epidemiology of Maternal Nutritional Status and Risk of Adverse Birth Outcomes in Undernourished Mothers with Sickle Cel... new window
Date: 2023-09-17 Authors: Klein LJ, Ayete-Nyampong JB, Williams AM, Harding LA, Oppong SA, Acra S, DeBaun MR, Imdad A. Journal: Methods and protocols - Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense ) and Camelina... new windowPennycress (Thlaspi arvense) and camelina (Camelina sativa) are nonfood winter oilseed crops that have the potential to contribute to sustainable biofuel production. However, undesired agronomic traits of pennycress and camelina currently hinder
Date: (2022) Authors: Minjeong Kang , Keunsub Lee , Kan Wang Ref: Protoplast Technology Date: 2023-09-15 Authors: Williams AE, Sanchez-Vargas I, Martin LE, Martin-Martin I, Bennett S, Olson KE, Calvo E. Journal: Journal of visualized experiments : JoVE - With the increasing understanding of fundamentals of gene silencing pathways in plants, various tools and techniques for downregulating the expression of a target gene have been developed across multiple plant species. This chapter provides an
Date: (2022) Authors: Prachi Pandey , Kirankumar S. Mysore , Muthappa Senthil-Kumar Ref: Plant Gene Silencing Date: 2023-09-13 Authors: Springer NA, Meyer SM, Taghavi A, Benhamou RI, Tong Y, Childs-Disney JL, Disney MD. Journal: Methods in enzymology