Results 741-750 of about 1,000
- Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans new windowSingle-guide RNAs can target exogenous CRISPR–Cas proteins to unique DNA locations, enabling genetic tools that are efficient, specific and scalable. Here we show that short synthetic guide Piwi-interacting RNAs (piRNAs) (21-nucleotide sg-piRNAs)
Date: (2022) Authors: Monika Priyadarshini , Julie Zhouli Ni , Amhed M. Vargas-Velazquez , Sam Guoping Gu , Christian Frøkjær-Jensen - Antisense Oligonucleotides as a Tool for Prolonged Knockdown of Nuclear lncRNAs in Human Cell Lines. new window
Date: 2023-09-01 Authors: Montiel-Manriquez R, Castro-Hernández C, Arriaga-Canon C, Herrera LA. Journal: Journal of visualized experiments : JoVE - Gene therapy has started in the late 1980s as novel, clinically applicable therapeutic option. It revolutionized the treatment of genetic diseases with the initial intent to repair or replace defective genes. Gene therapy has been adapted for
Date: (2022) Authors: Dennis Kobelt , Jessica Pahle , Wolfgang Walther Ref: Gene Therapy of Cancer Date: 2023-09-01 Authors: Sun R, Raban R, Akbari OS. Journal: Cold Spring Harbor protocols - Clostridium cellulolyticum is a model mesophilic, cellulolytic bacterium, with the potential to produce biofuels from lignocellulose. However, the natural cellulose utilization efficiency is quite low and, therefore, metabolically engineered strains
Date: (2022) Authors: Tao Xu , Xuanyu Tao , Megan L. Kempher , Jizhong Zhou Ref: Recombineering - Techniques for disrupting gene expression are invaluable tools for the analysis of the biological role of a gene product. Because of its genetic tractability and multiple advantages over conventional mammalian models, the zebrafish (Danio rerio) is
Date: (2022) Authors: Chiguang Feng , Mihai Nita-Lazar , Nuria González-Montalbán , Jingyu Wang ... Gerardo R. Vasta Ref: Galectins - Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling. new window
Date: 2023-08-26 Authors: Tsuchiya M, Tachibana N, Hamachi I. Journal: STAR protocols - Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq new windowPooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic interrogation of gene function and regulatory networks. Here, we introduce Cas13 RNA Perturb-seq (CaRPool-seq), which leverages the RNA-targeting CRISPR–Cas13d
Date: (2022) Authors: Hans-Hermann Wessels , Alejandro Méndez-Mancilla , Yuhan Hao , Efthymia Papalexi ... Rahul Satija - Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Periph... new window
Date: 2023-08-25 Authors: Turner-Ivey B, Longo JF, Jenkins DP, Guest ST, Carroll SL. Journal: Journal of visualized experiments : JoVE - The production of genetically modified mice and their behavioral analysis are important for evaluating the roles of genes and protein residues in regulating the mammalian circadian system at the organism level. However, the time and space required to
Date: (2022) Authors: Hideki Ukai , Koji L. Ode , Hiroki R. Ueda Ref: Circadian Clocks