Results 751-760 of about 1,000
- Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations. new window
Date: 2023-08-15 Authors: Merenciano M, Aguilera L, González J. Journal: STAR protocols - Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis new windowWith a surprisingly complex genome and an ever-expanding genetic toolkit, the sea anemone Nematostella vectensis has become a powerful model system for the study of both development and whole-body regenerationWhole-body regeneration (WBR). Here we
Date: (2022) Authors: Eric M. Hill , Cheng-Yi Chen , Florencia del Viso , Lacey R. Ellington ... Matthew C. Gibson Ref: Whole-Body Regeneration - Protocol to optimize the biobanking of ovarian cancer organoids by accommodating patient-specific differences in stemnes... new window
Date: 2023-08-15 Authors: Trillsch F, Czogalla B, Kraus F, Burges A, Mahner S, Kessler M. Journal: STAR protocols - Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells new windowFluorescence-activated cell sorting (FACS) allows for the enrichment of specific plant cell populations after protoplasting. In this book chapter, we describe the transformation and protoplasting of an Arabidopsis thaliana cell suspension culture
Date: (2022) Authors: Ward Decaestecker , Norbert Bollier , Rafael Andrade Buono , Moritz K. Nowack , Thomas B. Jacobs Ref: Protoplast Technology - Generation of epitope tag knock-in mice with CRISPR-Cas9 to study the function of endogenous proteins. new window
Date: 2023-08-15 Authors: Zhang Z. Journal: STAR protocols - Gene drives are genetic elements that are transmitted to greater than 50% of offspring and have potential for population modification or suppression. While gene drives are known to occur naturally, the recent emergence of CRISPR-Cas9 genome-editing
Date: (2022) Authors: Mark D. Bunting , Chandran Pfitzner , Luke Gierus , Melissa White ... Paul Q. Thomas Ref: Applications of Genome Modulation and Editing - High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes. new window
Date: 2023-08-04 Authors: Craft J, Truong T, Penn BH. Journal: Journal of visualized experiments : JoVE - Cloning Strategies for the Generation of Recombinant Capripoxvirus Through the Use of Screening and Selection Markers new windowThe ability to manipulate capripoxvirusCapripoxvirus through gene knockouts and gene insertions has become an increasingly valuable research tool in elucidating the function of individual genes of capripoxvirusCapripoxvirus, as well as in the
Date: (2022) Authors: Hani Boshra , Mahder Teffera , Jinxing Cao , Shawn Babiuk Ref: Vaccine Technologies for Veterinary Viral Diseases - Rapid Genotyping of Nothobranchius furzeri Embryos, Larvae, and Adult Fish via High-Resolution Melt Analysis (HRM... new window
Date: 2023-08-01 Authors: Krug J, Richter A, Englert C. Journal: Cold Spring Harbor protocols - In livestock industry, one sex is usually preferred over the other due to its impact on the production (e.g., milk from cows, eggs from laying hens, or meat from bulls). Boar taint, to which most of the consumers are susceptible, is a major challenge
Date: (2022) Authors: Björn Petersen , Stefanie Kurtz Ref: Applications of Genome Modulation and Editing