Results 101-110 of about 1,000
  1. High-Efficiency Cas12a-Mediated A-to-G Base Editing in Rice new window
    The development of CRISPR-Cas genome editing technology, notably the Cas9 and Cas12a systems, has significantly advanced biomedical studies and plant research. The Cas9-derived base editors have been effectively utilized for precise C-to-T, A-to-G,
    Date: (2025)
    Authors: Yanhao Cheng , Yiping Qi
    Ref: CRISPR-Cas Methods
    https://experiments.springernature.com/.../978-1-0716-4358-7_10 - SpringerNature
  2. Determining the biochemical function of type IV CRISPR ribonucleoprotein complexes and accessory proteins. new window
    Date: 2025-02-11
    Authors: Williams AA, Redman O, Domgaard H, Armbrust MJ, Jackson RN.
    Journal: Methods in enzymology
    https://doi.org/10.1016/bs.mie.2025.01.039 - Europe PMC
  3. Generating stably-expressing Cas9 cancer organoid lines new window
    Authors: "Jade Smith","Tessa Fowler","Agnieszka Andres","Adam Jackson","Emily Souster","Hazel Rogers","Alexandra Beck","Charlotte Beaver","Mathew Garnett",
    https://www.protocols.io/...expressing-cas9-cancer-organoid-dd3728rn - protocols.io
  4. Rapid Generation of Recombinant Poxviruses Using CRISPR/Cas9 Gene Editing new window
    The low-frequency natural recombination that is detected in poxvirus-infected cells has long been used to genetically modify poxviruses. Such recombinant poxviruses have found many applications as vaccines for preventing infectious diseases and as
    Date: (2025)
    Authors: Yi-Chan J. Lin , David H. Evans , Ryan S. Noyce
    Ref: Vaccinia, Mpox, and Other Poxviruses
    https://experiments.springernature.com/.../978-1-0716-4160-6_7 - SpringerNature
  5. Protocol for the purification and analysis of nuclear UFMylated proteins. new window
    Date: 2025-02-10
    Authors: Panichnantakul P, Oeffinger M.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2025.103634 - Europe PMC
  6. Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic... new window
    Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) is a new approach for ex vivo genome editing of primary human cells. PERC uses a single amphiphilic peptide reagent to mediate intracellular delivery of the same pre-formed
    Date: (2025)
    Authors: Srishti U. Sahu , Madalena Castro , Joseph J. Muldoon , Kunica Asija ... Ross C. Wilson
    https://experiments.springernature.com/.../s41596-025-01154-8 - SpringerNature
  7. Cloning and validating systems for high throughput molecular recording. new window
    Date: 2025-02-09
    Authors: Zhao A, Chan MM.
    Journal: Methods in enzymology
    https://doi.org/10.1016/bs.mie.2025.01.015 - Europe PMC
  8. Whole-genome CRISPR screening of stably expressing Cas9 cancer organoid lines new window
    Authors: "Tessa Fowler","Jade Smith","Adam Jackson","Agnieszka Andres","Emily Souster","Hazel Rogers","Alexandra Beck","Charlotte Beaver","Mathew Garnett",
    https://www.protocols.io/...-screening-of-stably-expressing-ddxg27jw - protocols.io
  9. CRISPR-Mediated Library Screening of Gene-Knockout Cell Lines for Investigating Antiviral Innate Immunity new window
    The application of CRISPR-mediated library screening has fundamentally transformed functional genomics by revealing the complexity of virus-host interactions. This protocol describes the use of CRISPR-mediated library screening to identify key
    Date: (2025)
    Authors: Yangkun Shen , Zhihua Feng , Chunfu Zheng , Qi Chen
    Ref: Antiviral Innate Immunity
    https://experiments.springernature.com/.../978-1-0716-4108-8_7 - SpringerNature
  10. Purification and in vivo, cell-free, and in vitro characterization of CRISPR-Cas12a2. new window
    Date: 2025-02-07
    Authors: Schut FT, Hallmark T, Dmytrenko O, Jackson RN, Beisel CL.
    Journal: Methods in enzymology
    https://doi.org/10.1016/bs.mie.2025.01.032 - Europe PMC
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