Results 241-250 of about 1,000
- Chromosome Counting at Meiosis and Mitosis of Mouse Oocytes and Embryos Using Super-Resolution Live-Cell Imaging and CRI... new window
Date: 2025-01-01 Authors: Hatano Y, Yonezawa N, Tokoro M, Yao T, Yamagata K. Journal: Methods in molecular biology (Clifton, N.J.) Authors: "Richard T Timms","Elijah L Mena","Stephen J Elledge", - A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato new windowThe Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—Cas 9 (CRISPR-associated protein 9) is a robust DNA-encoded, RNA-mediated sequence-specific nuclease system widely used for genome editing of various plants. Although there are
Date: (2024) Authors: Valluri V. Satyavathi , Kunnappady Princy , Neha Gupta , Narasimha Rao Nizampatnam ... Yellamaraju Sreelakshmi Ref: Plant Functional Genomics Authors: "Nefeli Skoufou", - SEcuRe 2.0 – A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm new window
Authors: "Magdalena Wigger","Branko Zevnik","Simon E Tröder", - Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems new windowCRISPR-Cas effectors are powerful tools for genome and transcriptome targeting and editing. Naturally, these protein–RNA complexes are part of the microbial innate immune system, which emerged from the evolutionary arms race between microbes and
Date: (2024) Authors: Carolin Maja Gebhardt , Dominik Niopek Ref: Mammalian Synthetic Systems - Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has enabled rapid advances in genomic engineering and transcriptional regulation. Specifically, CRISPR interference (CRISPRi) system has been used to systematically
Date: (2024) Authors: Minjeong Kang , Kangsan Kim , Byung-Kwan Cho Ref: Synthetic Biology Date: 2025-01-01 Authors: Suñer IG, Singh SP. Journal: Methods in molecular biology (Clifton, N.J.) Authors: "wusj","schekman", - Rapid generation of homozygous fluorescent knock-in human cells using CRISPR–Cas9 genome editing and validation by autom... new windowWe previously described a protocol for genome engineering of mammalian cultured cells with clustered regularly interspaced short palindromic repeats and associated protein 9 (CRISPR–Cas9) to generate homozygous knock-ins of fluorescent tags into
Date: (2024) Authors: Andrea Callegari , Moritz Kueblbeck , Natalia Rosalía Morero , Beatriz Serrano-Solano , Jan Ellenberg