Results 271-280 of about 1,000
Date: 2025-01-01 Authors: Nishizawa-Yokoi A, Toki S. Journal: Methods in molecular biology (Clifton, N.J.) Authors: "michela.deleidi", - The integration of CRISPR/Cas9 genome editing techniques with organoid technology has revolutionized the field of tumor modeling, enabling the creation of diverse tumor models with distinct mutational profiles. This protocol details the application
Date: (2024) Authors: Carme Cortina , Adrià Cañellas-Socias Ref: Cancer Cell Dormancy - Linking phenotypes to genetic components has been an essential part of novel drug discovery, and screening methods have been widely employed to achieve such a goal. Screens can be conducted in either pooled or arrayed formats. Although arrayed
Date: (2024) Authors: Nihal Terzi Çizmecioglu Ref: Skin Stem Cells Authors: "Lilly van de Venn","Zacharias Kontarakis","Beeke Wienert", - CRISPR-Cas9 genome editing is a powerful tool for assessing the functional role of candidate genes. In vitro CRISPR/Cas9 screens have been used to rapidly assess the role of thousands of genes in the differentiation and function of immune
Date: (2024) Authors: Kumari Anupam , Brian J. Laidlaw Ref: Memory B-Cells - Cell–cell interactions are essential for the function and contextual regulation of biological tissues. We present a platform for high-throughput microfluidics-supported genetic screening of functional regulators of cell–cell interactions. Systematic
Date: (2024) Authors: Camilo Faust Akl , Mathias Linnerbauer , Zhaorong Li , Hong-Gyun Lee ... Francisco J. Quintana Date: 2025-01-01 Authors: Schnormeier AK, Budeus B. Journal: Methods in molecular biology (Clifton, N.J.) - MicroRNAs represent an interesting group of regulatory molecules with the unique ability of a single miRNA able to regulate the expression of potentially hundreds of target genes. In that regard, their utility has been demonstrated as a strategy to
Date: (2024) Authors: Kevin Kellner , Nga T. Lao , Niall Barron Ref: Recombinant Protein Expression in Mammalian Cells - Virally Induced CRISPR/Cas9-Based Knock-In of Fluorescent Albumin Allows Long-Term Visualization of Cerebral Circulation... new windowAlbumin, a protein primarily produced by liver hepatocytes, is the most abundant protein in blood plasma. We have previously engineered a liver-targeting adeno-associated viral vector (AAV) that expresses fluorescent protein-tagged albumin to
Date: (2024) Authors: Marta Vittani , Philip Aleksander Knak , Masahiro Fukuda , Masaki Nagao ... Hajime Hirase Ref: Fluorescence Imaging of the Brain