Results 281-290 of about 1,000
Date: 2025-01-01 Authors: Stukenberg D, Hoff J, Faber A, Becker A. Journal: Methods in molecular biology (Clifton, N.J.) - Manipulation of Proteostasis Networks in Transgenic ZAAT Zebrafish via CRISPR–Cas9 Gene Editing new windowThe CRISPR–Cas9 genome editing system is used to induce mutations in genes of interest resulting in the loss of functional protein. A transgenic zebrafish α1-antitrypsin deficiency (AATD) model displays an unusual phenotype, in that it lacks the
Date: (2024) Authors: Connie Fung , Lee B. Miles , Robert J. Bryson-Richardson , Phillip I. Bird Ref: Alpha-1 Antitrypsin - In recent years, targeted genome editing has emerged as an indispensable tool for creating animal models, facilitating a comprehensive exploration of the molecular mechanisms governing a myriad of biological processes. Within this scientific
Date: (2024) Authors: Manuel Sánchez-Martín , Fernando Sánchez-Sáez , Elena Llano , Alberto M. Pendás Ref: Meiosis - The placenta is the organ that dictates the reproductive outcome of mammalian pregnancy by supplying nutrients and oxygen to the developing fetus to sustain its normal growth. During early mammalian development, trophoblast cells are the earliest
Date: (2024) Authors: Paula Doria-Borrell , Maria Moya-Navamuel , Myriam Hemberger , Vicente Pérez-García Ref: Maternal Placental Interface - The vast number of unknown phage-encoded ORFan genes and limited insights into the genome organization of phages illustrate the need for efficient genome engineering tools to study bacteriophage genes in their natural context. In addition, there is
Date: (2024) Authors: Kaat Schroven , Marleen Voet , Rob Lavigne , Hanne Hendrix Ref: Phage Engineering and Analysis Date: 2025-01-01 Authors: Vegh P, Chapman E, Gilmour C, Fragkoudis R. Journal: Methods in molecular biology (Clifton, N.J.) - CRISPR-Cas9 is a powerful tool to interrogate gene function in a targeted and systematic manner. Although the technology has been scaled up for large-scale genetic screens in cell culture, similar scale screens in vivo have been extremely challenging
Date: (2024) Authors: Saba Parvez , Tejia Zhang , Randall T. Peterson Ref: Zebrafish - Dual-Luciferase Reporter Assay for Prescreening CRISPR (d)Cas9-Mediated Epigenetic Editing on a Plant Promoter Using Hum... new windowEpigenetic editing, also known as EpiEdit, offers an exciting way to control gene expression without altering the DNA sequence. In this study, we evaluate the application of EpiEdit to plant promoters, specifically the MLO (mildew locus o) gene
Date: (2024) Authors: Ann-Kathrin Hinrichs , Aline Koch , Antje M. Richter Ref: Plant Functional Genomics - CRISPR/Cas9 system is one of the most often utilized engineering tools for genome editing in many organisms including crop plants and presents great value in both basic and applied research. This is a preferred method because of its relative
Date: (2024) Authors: Chudamani Sharma Prakash , Ramanjulu Sunkar Ref: Plant Stress Tolerance - Genome-Wide CRISPR-Cas9 Screening for the Identification of Host Dependency Factors of Emerging Viruses new windowLike all the RNA viruses, Rift Valley fever virus (RVFV) encodes only few viral proteins and relies heavily on the host cellular machinery for productive infection. This dependence creates a potential “Achille’s heel” that may be exploited to develop
Date: (2024) Authors: Laurent Meertens , Laurine Couture , Ali Amara Ref: Rift Valley Fever Virus