Results 41-50 of about 1,000
- The field of peptide-mediated cargo delivery has seen an evolution in the last four decades, starting with the naturally derived transactivator of transcription (TAT) and penetratin cell-penetrating peptides (CPPs) and gradually evolving to
Date: (2025) Authors: Oskar Gustafsson , Samir Andaloussi , Joel Z. Nordin Ref: Cancer-Targeted Drug Delivery - Protocol for ecotropic pseudotyped lentiviral transduction of "murinized" human cells while decreasing risk of infection... new window
Date: 2025-03-21 Authors: Weber SLK, Heinzel T, Iyer-Bierhoff A. Journal: STAR protocols - Sensitive Small Molecule Aptasensing Based on Hybridization Chain Reaction and CRISPR-Cas12a Using a Portable 3D-Printed... new windowThe next-generation biosensing tools based on CRISPR-Cas have revolutionized molecular detection. A number of CRISPR-Cas-based biosensors have been reported for the detection of nucleic acid targets. The establishment of efficient methods for
Date: (2025) Authors: Long Ma , Yaru Li , Shuli Man Ref: CRISPR-Cas Methods - Protocol Development for CRISPR/Cas9 Knockout of the Anti-inflammatory Protein TNIP1 in HaCaT Keratinocytes. new window
Date: 2025-03-20 Authors: Carman LE, Samulevich ML, Aneskievich BJ. Journal: Methods in molecular biology (Clifton, N.J.) Authors: "Christopher Sansam", Authors: "Harper JW","jiuchun_zhang@hms.harvard.edu", - Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells new windowGenome editing has become an important aspect of Chinese hamster ovary (CHO) cell line engineering for improving the production of recombinant protein therapeutics. Currently, the engineering focus is directed toward expanding product diversity while
Date: (2025) Authors: Lise Marie Grav , Johan Blatt Rojek , Karen Julie Cour Karottki , Jae Seong Lee , Helene Faustrup Kildegaard Ref: Heterologous Protein Production in CHO Cells - Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cel... new window
Date: 2025-03-18 Authors: Matsui S, Shiley JR, Granitto M, Ludwig K, Buckley M, Koigi S, Mirizio G, Hu YC, Mayhew CN, Iwafuchi M. Journal: STAR protocols - Breaking the Glass Ceiling of Stable Genetic Transformation and Gene Editing in the Popular Pepper cv Cayenne new window
Authors: "Manoj Kumar","Dana Ayzenshtat","Gulzar A. Rather","Emmanuella Aisemberg","Alexander Goldshmidt","Samuel Bocobza", - Phage engineering is an emerging technology due to the promising potential application of phages in medical and biotechnological settings. Targeted phage mutagenesis tools are required to customize the phages for a specific application and generate,
Date: (2025) Authors: Nadiia Pozhydaieva , Katharina Höfer Ref: Golden Gate Cloning