Results 651-660 of about 1,000
- Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airwa... new window
Date: 2024-01-01 Authors: Zaragosi LE, Gouleau A, Delin M, Lebrigand K, Arguel MJ, Girard-Riboulleau C, Rios G, Redman E, Plaisant M, Waldmann R, Magnone V, Marcet B, Barbry P, Ponzio G. Journal: Methods in molecular biology (Clifton, N.J.) - Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene new windowThe myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as “double-muscling phenotype.” Disruption
Date: (2022) Authors: Peter Kalds , Martina Crispo , Chao Li , Laurent Tesson ... Alejo Menchaca Ref: Applications of Genome Modulation and Editing - Dissecting the Role of the Ccr4-Not Deadenylase Complex in Pluripotency and Differentiation. new window
Date: 2024-01-01 Authors: Wang X, Chen Q, Lackford B, Hu G. Journal: Methods in molecular biology (Clifton, N.J.) - Here we provide a detailed tutorial on CRISPR in vivo screening. Using the mouse as the model organism, we introduce a range of CRISPR tools and applications, delineate general considerations for ‘transplantation-based’ or ‘direct in vivo’ screening
Date: (2022) Authors: Christian J. Braun , Andrés Carbonell Adames , Dieter Saur , Roland Rad - Genetically engineered mouse models (GEMMs) transformed the study of organismal disease phenotypes but are limited by their lengthy generation in embryonic stem cells. Here, we describe methods for rapid and scalable genome engineering in somatic
Date: (2022) Authors: Thorsten Kaltenbacher , Jessica Löprich , Roman Maresch , Julia Weber ... Roland Rad - Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis Using Mobile-CRISPRi. new window
Date: 2024-01-01 Authors: Yu MA, Banta AB, Ward RD, Prasad NK, Kwon MS, Rosenberg OS, Peters JM. Journal: Methods in molecular biology (Clifton, N.J.) - Designing Genetically Engineered Mouse Models (GEMMs) Using CRISPR Mediated Genome Editing new windowGenetically engineered mouse models (GEMMs) are very powerful tools to study lineage hierarchy and cellular dynamics of stem cells in vivo. Stem cell behavior in various contexts such as development, normal homeostasis and diseases have been
Date: (2022) Authors: Jade Desjardins , Mitra Cowan , Yojiro Yamanaka Ref: Stem Cell Assays Date: 2024-01-01 Authors: Chen W, Ji Q. Journal: Methods in molecular biology (Clifton, N.J.) - Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular CRISPRa System new windowCRISPR-Cas regulators have provided an excellent toolbox to control gene expression due to the versatility of its components and the easy programming of the single guide RNA (sgRNA) to target DNA sequences. Included in this are CRISPR activation
Date: (2022) Authors: Maria Claudia Villegas Kcam , James Chappell Ref: Riboregulator Design and Analysis - Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations Via a tRNA-Based Multiplex sgRNA Expressi... new window
Date: 2024-01-01 Authors: Shiraki T, Kawakami K. Journal: Methods in molecular biology (Clifton, N.J.)