Results 1-10 of about 1,000
  1. C. neoformans Electroporation Transformation new window
    Authors: "Thomas Davies",
    https://www.protocols.io/...electroporation-transformation-ehdebb23f - protocols.io
  2. CRISPR/Cas9–Mediated Gene Knockout and Knockin Human iPSCs new window
    The realization of the full potential of human pluripotent stem cells (hPSCs), including human induced PSCs (iPSC), relies on the ability to precisely edit their genome in a locus-specific and multiplex manner. Clustered Regularly Interspaced Short
    Date: (2025)
    Authors: Paolo Petazzi , Pablo Menéndez , Ana Sevilla
    https://experiments.springernature.com/.../10.1007/7651_2020_337 - SpringerNature
  3. Optical pooled screening for the discovery of regulators of the alternative lengthening of telomeres pathway. new window
    Date: 2025-05-03
    Authors: Quintanilla I, Azeroglu B, Sagar MAK, Stracker TH, Denchi EL, Pegoraro G.
    Journal: Methods (San Diego, Calif.)
    https://doi.org/10.1016/j.ymeth.2025.05.001 - Europe PMC
  4. Human Primary Astrocyte (hPA) CRISPRa reprogramming protocol new window
    Authors: "Samuel Reisman","Charles Gersbach",
    https://www.protocols.io/...ocyte-hpa-crispra-reprogramming-d7rd9m26 - protocols.io
  5. CRISPR/Cas-Mediated Knock-in of Genetically Encoded Fluorescent Biosensors into the AAVS1 Locus of Human-Induced Pluripo... new window
    Genetically encoded fluorescent biosensors (GEFBs) enable researchers to visualize and quantify cellular processes in live cells. Induced pluripotent stem cells (iPSCs) can be genetically engineered to express GEFBs via integration into the
    Date: (2025)
    Authors: David Stellon , Minh Thuan Tran , Jana Talbot , Sueanne Chear ... Anthony L. Cook
    https://experiments.springernature.com/.../10.1007/7651_2021_422 - SpringerNature
  6. Protocol for in vivo CRISPR knockout screening of autophagy genes to identify breast cancer metastasis suppressors. new window
    Date: 2025-04-30
    Authors: Hao M, Lu P, Guan JL.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2025.103798 - Europe PMC
  7. Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology new window
    Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based tool kits greatly facilitate the investigation of gene function, especially gene editing and transcriptional regulation technology. Human trophoblast stem cell (hTSC) is a
    Date: (2025)
    Authors: Hao Wu , Yue Wang , Hongmei Wang
    https://experiments.springernature.com/.../10.1007/7651_2022_464 - SpringerNature
  8. CRISPR revolution: Unleashing precision pathogen detection to safeguard public health and food safety. new window
    Date: 2025-04-29
    Authors: Liberty JT, Bromage S, Peter E, Ihedioha OC, Alsalman FB, Odogwu TS.
    Journal: Methods (San Diego, Calif.)
    https://doi.org/10.1016/j.ymeth.2025.04.018 - Europe PMC
  9. CRISPR genome editing using a combined positive and negative selection system new window
    Authors: "Ishrya Sharma","Kerisa Hall","Shannon Moonah",
    https://www.protocols.io/...ng-using-a-combined-positive-an-du4v6yw6 - protocols.io
  10. Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System new window
    Induced pluripotent stem cells (iPSCs) generated from patients are a valuable tool for disease modelling, drug screening, and studying the functions of cell/tissue-specific genes. However, for this research, isogenic iPSC lines are important for
    Date: (2025)
    Authors: Vasanth Thamodaran , Sonam Rani , Shaji R. Velayudhan
    https://experiments.springernature.com/.../10.1007/7651_2021_348 - SpringerNature
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