Results 131-140 of about 1,000
- CRISPR RNA-targeting tools enable the visualization of native individual transcripts in living cells.
Date: (2025) Authors: Lei Tang Date: 2025-02-01 Authors: Grimm MS, Myhrvold C. Journal: Methods in enzymology Authors: "Yun Li", - Gene Knockout in the Developing Brain of Wild-Type Rodents by CRISPR In Utero Electroporation new windowCRISPR/Cas9 constructs can be delivered by in utero electroporation to knock out a gene of interest in neurons of the developing brain in wild-type rodents. This approach allows for high-throughput genetic screening, circuit-specific gene knockout,
Date: (2025) Authors: Andrea J. Romanowski , Ryan R. Richardson , Celine Plachez , Reha S. Erzurumlu , Alexandros Poulopoulos Ref: Synapse Development Date: 2025-01-31 Authors: Liu C, Cheng S, Zhu J, Zhou L, Chen J. Journal: Methods in enzymology - Application of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging tool... new window
Authors: "Bhanu Prakash Potlapalli","Joerg Fuchs","Twan Rutten","Armin Meister","Andreas Houben", - Combining Oligo Pools and Golden Gate Cloning to Create Protein Variant Libraries or Guide RNA Libraries for CRISPR Appl... new windowOligo pools are array-synthesized, user-defined mixtures of single-stranded oligonucleotides that can be used as a source of synthetic DNA for library cloning. While currently offering the most affordable source of synthetic DNA, oligo pools also
Date: (2025) Authors: Alicia Maciá Valero , Rianne C. Prins , Thijs Vroet , Sonja Billerbeck Ref: Golden Gate Cloning - Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells. new window
Date: 2025-01-31 Authors: Stewart C, Manriquez Roman C, Kenderian SS. Journal: Journal of visualized experiments : JoVE - We present a protocol to perform CRISPR/Cas9-mediated genome editing in the fission yeast Schizosaccharomyces pombe that does not require cloning and uses the fluoride exporter channel Fex1 as the selection marker. Transformation is typically carried
Date: (2025) Authors: Yuan Ren , Ronan Fernandez , Takumi Saito , Barbara Fujita ... Julien Berro Ref: Schizosaccharomyces pombe - Protocol for functional screening of CFTR-targeted genetic therapies in patient-derived organoids using DETECTOR deep-le... new window
Date: 2025-01-31 Authors: Bulcaen M, Liu RB, Gryspeert K, Thierie S, Ramalho AS, Vermeulen F, Casadevall I Solvas X, Carlon MS. Journal: STAR protocols