Results 141-150 of about 1,000
  1. Highly Efficient Genome Editing in Plants with the LbCas12a-RRV Variant new window
    Cas12a (previously known as Cpf1), belonging to the Class II type V CRISPR system, has gained extensive utilization in plant genome editing and has distinctive characteristics compared to Cas9. Despite being recognized as one of the most robust
    Date: (2025)
    Authors: Gen Li , Gary Coleman , Yiping Qi
    Ref: CRISPR-Cas Methods
    https://experiments.springernature.com/.../978-1-0716-4358-7_8 - SpringerNature
  2. Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish. new window
    Date: 2025-01-24
    Authors: Jin X, Zhang B, Sun Y, Duan Y, Lu J, Liu J, Han J, Zhang Y.
    Journal: Journal of visualized experiments : JoVE
    https://doi.org/10.3791/67616 - Europe PMC
  3. CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP new window
    Authors: "Jiuchun Zhang","Harper JW","Sharan Sharan Swarup",
    https://www.protocols.io/...he-tmem192-gene-with-3xha-in-h9-cx8axrse - protocols.io
  4. Efficient Single-Cell Cloning of Genome-Edited Cultured Human Cells new window
    To isolate human cell clones with intended genotypes induced by genome editing, a large number of clones must be isolated by single-cell cloning. Selection markers such as drug-resistant genes can be used to enhance the chance of isolation of target
    Date: (2025)
    Authors: Gou Takahashi , Yuichiro Miyaoka
    Ref: CRISPR-Cas Methods
    https://experiments.springernature.com/.../978-1-0716-4358-7_12 - SpringerNature
  5. Protocol for CRISPR-based endogenous protein tagging in mammalian cells. new window
    Date: 2025-01-20
    Authors: Xiao YX, Wei J, Moffat J.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2024.103571 - Europe PMC
  6. Generation of human fetal brain organoids and their CRISPR engineering for brain tumor modeling new window
    The developing human brain displays unique features that are difficult to study in animal models. Current in vitro models based on human brain tissue face several challenges, including the limited cellular heterogeneity in two- or three-dimensional
    Date: (2025)
    Authors: Anna Pagliaro , Francesco Andreatta , Roxy Finger , Benedetta Artegiani , Delilah Hendriks
    https://experiments.springernature.com/.../s41596-024-01107-7 - SpringerNature
  7. Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique. new window
    Date: 2025-01-14
    Authors: Yamamoto T, Urabe F, Yoshioka Y, Yamamoto Y, Ochiya T.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2024.103569 - Europe PMC
  8. CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP new window
    Authors: "Jiuchun Zhang","Harper JW",
    https://www.protocols.io/...he-eea1-gene-in-h9-es-cells-for-cx6yxrfw - protocols.io
  9. In Situ Phytomicrobiome Engineering new window
    This chapter explores the cutting-edge field of in situ phytomicrobiome engineering, employing advanced methodologies to enhance plant–microbe interactions for sustainable agriculture. This chapter delves into the integration of “omics” technologies,
    Date: (2025)
    Authors: Krishnan Ajith Kumar , Lokesh Ravi
    Ref: Plant Microbiome Engineering
    https://experiments.springernature.com/.../978-1-0716-4180-4_49 - SpringerNature
  10. Protocol for detecting eDNA in ecological rare fish using RPA-CRISPR-Cas12a technology. new window
    Date: 2025-01-10
    Authors: Wei XY, Pei Y, Liu L, Hamar P, Pei DS.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2024.103544 - Europe PMC
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