Results 291-300 of about 1,000
- CRISPR-Cas9 Technology in Plant Genomics Research: A Review and Tutorial for Implementation with Considerations for Orch... new windowThe study and identification of gene mutations resulting in alterations to plant phenotype form the basis of plant genetic screens. Assigning function to particular loci or genes based on mutated alleles is critical for breeding programs in order to
Date: (2024) Authors: Neil M. Hickerson , Marcus A. Samuel Ref: Orchid Propagation - Alteration of protein tyrosine phosphatase (PTP) gene expression is a commonly used approach to experimentally analyze their function in the cell physiology of mammalian cells. Here, exemplified for receptor-type PTPRJ (Dep-1, CD148) and PPTRC
Date: (2024) Authors: Carolin Lossius , Anne Kresinsky , Laura Quiet , Jörg P. Müller Ref: Protein Tyrosine Phosphatases - Protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid syste... new window
Date: 2024-12-21 Authors: Hu Y, Chen Y, Zhang Y, Liu Z, Li J. Journal: STAR protocols - Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells new windowGene editing in the murine germline is a valuable approach to investigate germ cell maturation and generate mouse models. Several studies demonstrated that CRISPR/Cas9 alters the genome of cultured male mouse germline stem cells delivered by
Date: (2024) Authors: Mariella Obermeier , Vera Rogiers , Tamara Vanhaecke , Yoni Baert Ref: Germ Cell Development Date: 2024-12-21 Authors: Su P, Miao YL. Journal: Methods in molecular biology (Clifton, N.J.) - Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications new windowIn traditional cell line design pipelines, cost- and time-intensive long-term stability studies must be performed due to random integration of the transgene into the genome. By this, integration into epigenetically silenced regions can lead to
Date: (2024) Authors: Oliver Hertel , Anne Neuss Ref: Recombinant Protein Expression in Mammalian Cells - Establishment of Stable Knockdown of MACC1 Oncogene in Patient-Derived Ovarian Cancer Organoids. new window
Date: 2024-12-20 Authors: Hierlmayer S, Hladchenko L, Reichenbach J, Klein C, Mahner S, Trillsch F, Kessler M, Chelariu-Raicu A. Journal: Methods and protocols - CRISPR screens are untangling molecular mechanisms that drive biological processes with greater precision and detail.
Date: (2024) Authors: Lei Tang Date: 2024-12-18 Authors: Eidelman M, Eisenberg E, Levanon EY. Journal: Methods in enzymology - Small RNAs (sRNAs) are versatile regulators universally present in species across the prokaryotic kingdom, yet their functional characterization remains a major bottleneck. Gene inactivation through random transposon insertion has proven extremely
Date: (2024) Authors: Gianluca Prezza , Alexander J. Westermann Ref: Bacterial Regulatory RNA