Results 331-340 of about 1,000
- Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils new windowEosinophils are a class of granulocytes with pleiotropic functions in homeostasis and various human diseases. Nevertheless, they are absent from conventional single-cell RNA sequencing atlases owing to technical difficulties preventing their
Date: (2024) Authors: Costanza Borrelli , Alessandra Gurtner , Isabelle C. Arnold , Andreas E. Moor - Protocol for Cas9-targeted long-read sequencing in Globodera pallida and Globodera rostochiensis. new window
Date: 2024-11-01 Authors: Sonawala U, Derevnina L, Eves-van den Akker S. Journal: STAR protocols - Osteoclasts are specialized cells that degrade bone and are essential for bone formation and maintaining bone homeostasis. Excess or deficient activity of these cells can significantly alter bone mass, structure, and physical strength, leading to
Date: (2024) Authors: Nina Reuven , Maayan Barnea-Zohar , Ari Elson Ref: Protein Tyrosine Phosphatases Date: 2024-10-30 Authors: Bao Y, Wei W. Journal: STAR protocols - Five distinct gene therapy approaches have been developed for treating AATD. These approaches include knockout of the mutant (PiZ) allele by introduction of double-strand breaks (DSBs) and subsequent creation of insertions and deletions (indels) by
Date: (2024) Authors: Alisha M. Gruntman , Wen Xue , Terence R. Flotte Ref: Alpha-1 Antitrypsin Date: 2024-10-24 Authors: Sorbara M, Cristol M, Cornebois A, Desrumeaux K, Cordelier P, Bery N. Journal: STAR protocols - A set of peroxidases detoxifies H2O2 and mediates H2O2-dependent signal propagation. The peroxidases include peroxiredoxins, glutathione peroxidases, ascorbate peroxidases, and catalases. This at least partial redundancy impedes addressing individual
Date: (2024) Authors: Lara Vogelsang , Karl-Josef Dietz Ref: ROS Signaling in Plants - Protocol for the derivation and culture of murine trophoblast organoids for CRISPR-Cas9 screening. new window
Date: 2024-10-19 Authors: Mao Q, Jiang J, Ye Q, Wang H, Lin CP. Journal: STAR protocols - Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs new windowTransposon (IS200/IS605)-encoded TnpB proteins are predecessors of class 2 type V CRISPR effectors and have emerged as one of the most compact genome editors identified thus far. Here, we optimized the design of Deinococcus radiodurans (ISDra2) TnpB
Date: (2024) Authors: Kim Fabiano Marquart , Nicolas Mathis , Amina Mollaysa , Saphira Müller ... Gerald Schwank - Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to mammalian cells. Amongst all editing approaches, prime editing (PE) has the unique potential to perform
Date: (2024) Authors: Francesco Aulicino , Renata A. Raele , Alexandra Harrison , Imre Berger Ref: Baculovirus