Results 401-410 of about 1,000
- SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRI... new windowA wide range of diseases, including cancer, autoimmune diseases, or neurodegenerative diseases, have been associated with single nucleotide mutations in their causative genes. Clustered regularly interspaced short palindromic
Date: (2023) Authors: Shohei Maruyama , Takashi Kusakabe , Xinyi Zou , Yoshiaki Kobayashi ... Kumiko Ui-Tei Ref: Genome Editing in Animals - Protocol for the characterization of the pancreatic tumor microenvironment using organoid-derived mouse models and singl... new window
Date: 2024-07-24 Authors: Jihad M, Mucciolo G, Li W, Anand A, Araos Henríquez J, Pinto Teles S, Manansala JS, Ashworth S, Lloyd EG, Cheng PSW, Luo W, Sawle A, Piskorz A, Biffi G. Journal: STAR protocols - A new Bayesian factor analysis method improves detection of genes and biological processes affected by perturbations in ... new windowClustered regularly interspaced short palindromic repeats (CRISPR) screening coupled with single-cell RNA sequencing has emerged as a powerful tool to characterize the effects of genetic perturbations on the whole transcriptome at a single-cell
Date: (2023) Authors: Yifan Zhou , Kaixuan Luo , Lifan Liang , Mengjie Chen , Xin He - Rapid and one-tube detection of human metapneumovirus using the RT-RPA and CRISPR/Cas12a. new window
Date: 2024-07-20 Authors: Du Y, Liu X, Gao H, Liu X, Huang M, Chai Q, Xing Z, Zhang T, Ma D. Journal: Journal of virological methods - The clustered regularly interspaced short palindromic repeats (CRISPR)-Caspase9 (Cas9) system provides a programmable technology that may be used to edit the eukaryotic genome and epigenome. CRISPR/Cas9 includes a guide RNA targeted to a gene of
Date: (2023) Authors: Christine L. Xu , Merry Zhechao Ruan , Sara D. Ragi , Stephen H. Tsang Ref: Retinitis Pigmentosa - Protocol for the derivation and alveolar type 2 differentiation of late-stage lung tip progenitors from the developing h... new window
Date: 2024-07-18 Authors: Lim K, Rawlins EL. Journal: STAR protocols - Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Impr... new windowCRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) has become a breeding tool of choice for eliciting targeted genetic alterations in crop species as a means of improving a wide range of agronomic
Date: (2023) Authors: Udaya Subedi , Kimberley Burton Hughes , Guanqun Chen , Abdelali Hannoufa , Stacy D. Singer Ref: Plant-Pathogen Interactions - Protocol to create isogenic disease models from adult stem cell-derived organoids using next-generation CRISPR tools. new window
Date: 2024-07-12 Authors: Celotti M, Derks LLM, van Es J, van Boxtel R, Clevers H, Geurts MH. Journal: STAR protocols - Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line new windowClustered regularly interspaced short palindromic repeats (CRISPR) technology is a powerful genome editing tool. Recently developed CRISPR-Cas12a system confers several advantages over CRISPR-Cas9, making it ideal for use in plant genome editing and
Date: (2023) Authors: Hong Fang , James N. Culver , Randall P. Niedz , Yiping Qi Ref: Plant Genome Engineering - Protocol to use protein language models predicting and following experimental validation of function-enhancing variants ... new window
Date: 2024-07-12 Authors: He Y, Zhou X, Yuan F, Chang X. Journal: STAR protocols