Results 511-520 of about 1,000
- Comprehensive spatiotemporal mapping of single-cell lineages in developing mouse brain by CRISPR-based barcoding new windowA fundamental interest in developmental neuroscience lies in the ability to map the complete single-cell lineages within the brain. To this end, we developed a CRISPR editing-based lineage-specific tracing (CREST) method for clonal tracing in Cre
Date: (2023) Authors: Lianshun Xie , Hengxin Liu , Zhiwen You , Luyue Wang ... Yuejun Chen - Generation of Cell Lines Stably Expressing a dCas9-Fusion or sgRNA to Address Dynamics of Long-Term Effects of Epigeneti... new window
Date: 2024-01-01 Authors: Sarno F, Koncz M, Eilers RE, Verschure PJ, Rots MG. Journal: Methods in molecular biology (Clifton, N.J.) - Floxed alleles and Cre drivers are two components of most conditional knockout mouse models, which are not only important for studying a given gene in a tissue-specific manner, but also useful for functional analysis of various sized genomic regions.
Date: (2023) Authors: Mia Wallace , J. Michael White , Evgenea Kouranova , Zi Teng Wang , Xiaoxia Cui Ref: Transgenesis - A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virothera... new windowOncolytic virotherapy represents an efficient immunotherapeutic approach for cancer treatment. Oncolytic viruses (OVs) promote antitumor responses through tumor-selective cell lysis and immune system activation. However, some tumor cell lines and
Date: (2023) Authors: Michela Muscolini , John Hiscott , Evelyne Tassone Ref: HDAC/HAT Function Assessment and Inhibitor Development - Plasmid Delivery and Single-Cell Plasmid Expression Analysis for CRISPR/dCas9-Based Epigenetic Editing. new window
Date: 2024-01-01 Authors: van den Berg van Saparoea ACH, van Loosen QC, Sarno F, Ntini E, Rots MG, Gjaltema RAF, Verschure PJ. Journal: Methods in molecular biology (Clifton, N.J.) - Androglobin (ADGB), the most recently identified member of the mammalian globin family, is a chimeric protein with an unusual, embedded globin domain that is circularly permutated and exhibits hallmarks of a hexacoordinated heme-binding scheme.
Date: (2023) Authors: Teng Wei Koay , Johannes Schödel , David Hoogewijs Ref: Oxygen Sensing - Currently methods for generating soybean edited lines are time-consuming, inefficient, and limited to certain genotypes. Here we describe a fast and highly efficient genome editing method based on CRISPR-Cas12a nuclease system in soybean. The method
Date: (2023) Authors: Dawei Liang , Yubo Liu , Chao Li , Qin Wen ... Liang Shi Ref: Plant Genome Engineering Date: 2024-01-01 Authors: Sekita Y, Kimura T. Journal: Methods in molecular biology (Clifton, N.J.) - CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line new windowThe protocol in this chapter describes a method to label endogenous proteins using a self-complementing split green fluorescent protein (split GFP1-10/11) in a human cell line. By directly delivering Cas9/sgRNA ribonucleoprotein (RNP) complexes
Date: (2023) Authors: Ryo Tamura , Daichi Kamiyama Ref: Fluorescent Proteins - Protocol for Efficient Generation of Chimeric Antigen Receptor T Cells with Multiplexed Gene Silencing by Epigenome Edit... new window
Date: 2024-01-01 Authors: Azcona MSR, Mussolino C. Journal: Methods in molecular biology (Clifton, N.J.)