Results 621-630 of about 1,000
- Streptomyces are an important source and reservoir of natural products with diverse applications in medicine, agriculture, and food. Engineered Streptomyces strains have also proven to be functional chassis for the discovery and production of
Date: (2022) Authors: Lee Ling Tan , Elena Heng , Nadiah Zulkarnain , Wan-Chi Hsiao ... Mingzi M. Zhang Ref: Recombineering - Enzyme-free targeted DNA demethylation using CRISPR–dCas9-based steric hindrance to identify DNA methylation marks causa... new windowDNA methylation involves the enzymatic addition of a methyl group primarily to cytosine residues in DNA. This protocol describes how to produce complete and minimally confounded DNA demethylation of specific sites in the genome of cultured cells by
Date: (2022) Authors: Daniel M. Sapozhnikov , Moshe Szyf Date: 2024-01-01 Authors: Liu S. Journal: Methods in molecular biology (Clifton, N.J.) - Genetic screens are a classic approach to dissecting biological pathways including membrane trafficking. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 have enabled the utility of this approach in diploid models, including
Date: (2022) Authors: Daniel R. Gulbranson Ref: Membrane Trafficking - The rise of the clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) system has made it possible to induce double-strand breaks at almost any desired target site in the genome. In plant somatic cells,
Date: (2022) Authors: Michelle Rönspies , Patrick Schindele , Rebecca Wetzel , Holger Puchta - Manipulating mRNA-binding protein Cth2 function in budding yeast Saccharomyces cerevisiae. new window
Date: 2024-01-01 Authors: Patnaik PK, Barlit H, Labunskyy VM. Journal: STAR protocols - The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe our most up-to-date protocols using the
Date: (2022) Authors: Charlotte J. Martin , John A. Calarco Ref: C. elegans Date: 2024-01-01 Authors: Bremer HJ, Pflum MKH. Journal: Methods in molecular biology (Clifton, N.J.) - Targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become an established method to introduce changes into a plant genome. While traditional genetic modification allows us to follow
Date: (2022) Authors: Johann Schernthaner , Margaret Balcerzak , Jhadeswar Murmu , Rajagopal Subramaniam Ref: Accelerated Breeding of Cereal Crops Date: 2024-01-01 Authors: Reuven N, Barnea-Zohar M, Elson A. Journal: Methods in molecular biology (Clifton, N.J.)