Results 641-650 of about 1,000
- The recent COVID-19 outbreak and pandemic of 2020 and its surveillance were implemented by quickly adapting the existing diagnostic methods to detect the SARS-CoV-2 RNA. While traditional methods for detecting pathogenic DNA and RNA have relied
Date: (2022) Authors: Rhythm Phutela , Sneha Gulati , Manoj Kumar , Souvik Maiti , Debojyoti Chakraborty Ref: Multiplex Biomarker Techniques - Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or clustered regularly interspaced
Date: (2022) Authors: Irene Santos-Barriopedro , Guido Mierlo , Michiel Vermeulen - The last two decades have marked significant advancement in the genome editing field. Three generations of programmable nucleases (ZFNs, TALENs, and CRISPR-Cas system) have been adopted to introduce targeted DNA double-strand breaks (DSBs) in
Date: (2022) Authors: Yasaman Shamshirgaran , Jun Liu , Huseyin Sumer , Paul J. Verma , Amir Taheri-Ghahfarokhi Ref: Applications of Genome Modulation and Editing - Optimization of Vectors and Targeting Strategies Including GoldenBraid and Genome Editing Tools: GoldenBraid Assembly of... new windowNew breeding techniques, especially CRISPR/Cas, could facilitate the expansion and diversification of molecular farming crops by speeding up the introduction of new traits that improve their value as biofactories. One of the main advantages of
Date: (2022) Authors: Beatriz González , Marta Vazquez-Vilar , Javier Sánchez-Vicente , Diego Orzáez Ref: Recombinant Proteins in Plants - CRISPR/Cas9 Mutagenesis to Generate Novel Traits in Bactrocera tryoni for Sterile Insect Technique new windowSterile Insect Technique (SIT) is a biocontrol strategy that has been widely utilized to suppress or eradicate outbreak populations of insect pests such as tephritid fruit flies. As SIT is highly favored due to it being species-specific and
Date: (2022) Authors: Amanda Choo , Elisabeth Fung , Thu N. Nguyen , Anzu Okada , Peter Crisp Ref: Applications of Genome Modulation and Editing Date: 2024-01-01 Authors: Nagalakshmi U, Meier N, Dinesh-Kumar SP. Journal: Methods in molecular biology (Clifton, N.J.) - CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-β Pathway Regulators new windowThe CRISPR/Cas technology has revolutionized forward genetic screening, and thereby facilitated genetic dissection of cellular processes and pathways. TGF-β signaling is a highly conserved cascade involved in development, regeneration, and diseases
Date: (2022) Authors: Nina Frey , Gerald Schwank Ref: TGF-Beta Signaling - In the presence of different physiological and environmental stresses, cells rapidly initiate stress responses to re-establish cellular homeostasis. Stress responses usually orchestrate both transcriptional and translational programs via distinct
Date: (2022) Authors: Xiaoyan Guo , Martin Kampmann Ref: The Integrated Stress Response - A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultu... new window
Date: 2024-01-01 Authors: Turan FB, Ercan ME, Firat-Karalar EN. Journal: Methods in molecular biology (Clifton, N.J.) - Identification and Validation of CRISPR/Cas9 Off-Target Activity in Hematopoietic Stem and Progenitor Cells new windowTargeted genome editing in hematopoietic stem and progenitor cells (HSPCs) using CRISPR/Cas9 can potentially provide a permanent cure for hematologic diseases. However, the utility of CRISPR/Cas9 systems for therapeutic genome editing can be
Date: (2022) Authors: So Hyun Park , Ciaran M. Lee , Gang Bao Ref: Stem Cell Assays