Results 771-780 of about 1,000
Date: 2023-07-09 Authors: Zeng X, Wang S, Liang M, Wang W, Jiang Y, Xu F, Liu L, Yan H, Tong Y, Zhang L, Tan GY. Journal: STAR protocols - Researchers describe Retro-Cascorder, a tool that employs prokaryotic retrons and CRISPR integrases to write transcriptional events into the genome of Escherichia coli.
Date: (2022) Authors: Lei Tang - A simple approach to improving RNA synthesis: Salt inhibition of RNA rebinding coupled with strengthening promoter bindi... new window
Date: 2023-07-07 Authors: Malagoda Pathiranage K, Martin CT. Journal: Methods in enzymology - Live-cell fluorescence microscopy is an effective tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors and after RNA interference-mediated or CRISPR knockout-mediated depletion of protein targets. Live
Date: (2022) Authors: John R. Daum , Casey O. DuBose , Sushama Sivakumar , Gary J. Gorbsky Ref: Mitosis Date: 2023-07-07 Authors: Friedman-DeLuca M, Patel PP, Karadal-Ferrena B, Barth ND, Duran CL, Ye X, Papanicolaou M, Condeelis JS, Oktay MH, Borriello L, Entenberg D. Journal: Journal of visualized experiments : JoVE - CRISPR-based detection and recording technologies are gaining increasing attention in disease surveillance and prevention. In this chapter, we describe how our recent discovery of noncanonical crRNAs inspired the engineering of reprogrammed tracrRNAs
Date: (2022) Authors: Chunlei Jiao , Chase L. Beisel Ref: Riboregulator Design and Analysis - Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells. new window
Date: 2023-07-07 Authors: Geyer F, Geyer M, Klapproth S, Wolff KD, Nieberler M. Journal: STAR protocols - As the genetic mutations driving human disease are identified, there is an increasing need for a biomedical model that can accurately represent the disease of interest and provide a platform for potential therapeutic testing. Pigs are a better model
Date: (2022) Authors: Taylor Goldsmith , Alla Bondareva , Dennis Webster , Anna Laura Voigt ... Ina Dobrinski Ref: Applications of Genome Modulation and Editing - Protocols for transgenesis at a safe harbor site in the Xenopus laevis genome using CRISPR-Cas9. new window
Date: 2023-06-28 Authors: Shibata Y, Okumura A, Mochii M, Suzuki KT. Journal: STAR protocols - Functionalized Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations new windowThe ability to track and isolate unique cell lineages from large heterogeneous populations increases the resolution at which cellular processes can be understood under normal and pathogenic states beyond snapshots obtained from single-cell RNA
Date: (2022) Authors: Andrea Gardner , Daylin Morgan , Aziz Al’Khafaji , Amy Brock Ref: Biomedical Engineering Technologies