Results 321-330 of about 1,000
- Recombinant mucins are attractive polymeric building blocks for new biomaterials, biolubricants, and therapeutics. Advances in glycoengineered host cell systems now enable the recombinant production of mucins with tailored O-glycan side chains,
Date: (2024) Authors: Ling-Ting Huang , Marshall J. Colville , Matthew Paszek Ref: Mucins - Protocol for isolating specific C. elegans neuron types for bulk and single-cell RNA sequencing. new window
Date: 2024-11-06 Authors: Taylor SR, McWhirter RD, Matlock BK, Flaherty DK, Miller DM. Journal: STAR protocols - CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes
Date: (2024) Authors: Stefanie Kuhns , Alice Dupont Juhl , Zeinab Anvarian , Daniel Wüstner ... Jens S. Andersen Ref: Cilia - Protocol for transformation-free genome editing in plants using RNA virus vectors for CRISPR-Cas delivery. new window
Date: 2024-11-05 Authors: Lou H, Xiang H, Zeng W, Jiang J, Zhang J, Xu L, Zhao C, Gao Q, Li Z. Journal: STAR protocols - Macrophages represent a broad spectrum of distinct, but closely related tissue-resident immune cells. This presents a major challenge for the study of functional aspects of these cells using classical Cre recombinase-mediated conditional mutagenesis
Date: (2024) Authors: Sigalit Boura-Halfon , Rebecca Haffner-Krausz , Shifra Ben-Dor , Jung-Seok Kim , Steffen Jung Ref: Tissue-Resident Macrophages Date: 2024-11-02 Authors: Mamatha Bhanu LS, Kataki S, Chatterjee S. Journal: Journal of microbiological methods - Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. We provide a
Date: (2024) Authors: Emily Montal , Shruthy Suresh , Yilun Ma , Mohita M. Tagore , Richard M. White Ref: Zebrafish Date: 2024-11-01 Authors: Inen J, Han CM, Farrel DM, Bilousova G, Kogut I. Journal: Journal of visualized experiments : JoVE - Cell Cycle–Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modified Cell Lines new windowThe identification of protein phosphatase 1 (PP1) holoenzyme substrates has proven to be a challenging task. PP1 can form different holoenzyme complexes with a variety of regulatory subunits, and many of those are cell cycle regulated. Although
Date: (2024) Authors: Dorothee C Kommer , Konstantinos Stamatiou , Paola Vagnarelli Ref: Cell Cycle Control - CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions. new window
Date: 2024-11-01 Authors: Sheikh MA, Afandi FH, Iannello G, Corneo B, Emerald BS, Ansari SA. Journal: Journal of visualized experiments : JoVE