Results 391-400 of about 1,000
- Protocol for generation and engineering of thyroid cell lineages using CRISPR-Cas9 editing to recapitulate thyroid cance... new window
Date: 2024-08-10 Authors: Pantina VD, Verona F, Turdo A, Veschi V, Modica C, Lo Iacono M, Gaggianesi M, Di Bella S, Todaro M, Di Franco S, Stassi G. Journal: STAR protocols - Pandemic countermeasures require the rapid design of antigens for vaccines, profiling patient antibody responses, assessing antigen structure-function landscapes, and the surveillance of emerging viral lineages. Cell surface display of a viral
Date: (2024) Authors: Andrea Quezada , Ankur Annapareddy , Kamyab Javanmardi , John Cooper , Ilya J. Finkelstein Ref: Recombinant Glycoproteins - Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay new windowTo integrate with host chromatin and establish a productive infection, HIV-1 must translocate the viral Ribonucleoprotein (RNP) complex through the nuclear pore complex (NPC). Current assay to measure HIV-1 nuclear import relies on a transient
Date: (2024) Authors: Adarsh Dharan , Edward M. Campbell Ref: HIV Protocols Date: 2024-07-29 Authors: Martinez P, Patel H, You Y, Doud EH, Mosley AL, Lasagna-Reeves CA. Journal: STAR protocols - CRISPR/Cas has been established for targeted mutagenesis in many plant species since 2013, including Brassica napus and Brassica oleracea. Since that time, improvements have been made in terms of efficiency and choice of CRISPR systems. This protocol
Date: (2023) Authors: Tom Lawrenson , Mark Youles , Monika Chhetry , Martha Clarke ... Penny Hundleby Ref: Plant Genome Engineering - Application of bioluminescence resonance energy transfer assays in primary mouse neuronal cultures. new window
Date: 2024-07-27 Authors: Hoang HTM, George K, Ahmad M. Journal: STAR protocols - Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins new windowRNA molecules perform various crucial roles in diverse cellular processes, from translating genetic information to decoding the genome, regulating gene expression and catalyzing chemical reactions. RNA-binding proteins (RBPs) play an essential role
Date: (2023) Authors: Renmei Liu , Jing Yao , Siyu Zhou , Jing Yang ... Xianjun Chen - Protocol for constructing tumor-targeting ANM-PROTACs for degradation of transcription factors. new window
Date: 2024-07-27 Authors: Li J, Liang C. Journal: STAR protocols - CRISPR-Cas9 Correction of Duchenne Muscular Dystrophy in Mice by a Self-Complementary AAV Delivery System new windowDuchenne muscular dystrophy (DMD) is a fatal neuromuscular disorder, caused by mutations in the DMD gene coding dystrophin. Applying clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (CRISPR-Cas) for
Date: (2023) Authors: Yu Zhang , Rhonda Bassel-Duby , Eric N. Olson Ref: Muscular Dystrophy Therapeutics Date: 2024-07-25 Authors: Luo J, Lu C, Yang X. Journal: STAR protocols