Results 451-460 of about 1,000
Date: 2024-04-17 Authors: Vora DS, Bhandari SM, Sundar D. Journal: Methods (San Diego, Calif.) - Many genetically engineered rat strains have been produced by the development of genome editing technology, although it used to be technical difficulty and low production efficiency. Knockout and knock-in strains can be simple and quick produced
Date: (2023) Authors: Takehito Kaneko Ref: Genome Editing in Animals - Protocol for efficient CRISPR-Cas9-mediated fluorescent tag knockin in hard-to-transfect erythroid cell lines. new window
Date: 2024-04-17 Authors: Deleuze V, Soler E, Andrieu-Soler C. Journal: STAR protocols - CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple
Date: (2023) Authors: Carlos Sánchez-Gómez , David Posé , Carmen Martín-Pizarro Ref: Polyploidy - Gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. new window
Date: 2024-04-15 Authors: Mühlgrabner V, Plach A, Holler J, Leitner J, Steinberger P, Dupré L, Göhring J, Huppa JB. Journal: Methods in cell biology - We are advancing a novel strategy for the treatment of solid tumors by employing CRISPR-directed gene editing to reduce levels of standard of care required to halt or reverse the progression of tumor growth. We intend to do this by utilizing a
Date: (2023) Authors: Natalia Rivera-Torres , Pawel Bialk , Eric B. Kmiec Ref: Cancer Systems and Integrative Biology Date: 2024-04-14 Authors: Erhardt V, Snow CM, Hosur V. Journal: STAR protocols - The CRISPR–Cas gene editing tools are fantastic examples of drawing inspiration from nature. Since their debut almost a decade ago, scientists have made various improvements to these gene editors, making them more versatile, more efficient and more
Date: (2023) Authors: Hui Hua - Protocol for genomic recombineering in Yersinia ruckeri using CRISPR Cas12a coupled with the λ Red system. new window
Date: 2024-04-12 Authors: Ejaz RN, Taylor NMI, Steiner-Rebrova EM. Journal: STAR protocols - Discovery of off-target CRISPR–Cas activity in patient-derived cells and animal models is crucial for genome editing applications, but currently exhibits low sensitivity. We demonstrate that inhibition of DNA-dependent protein kinase catalytic
Date: (2023) Authors: Roger S. Zou , Yang Liu , Oscar E. Gaido , Maximilian F. Konig ... Taekjip Ha