Results 471-480 of about 1,000
- Protocol for the generation and purification of high-molecular-weight covalent RNA-DNA hybrids with T4 RNA ligase new window
Date: 2024-03-01 Authors: Laguía O, Bosso G, Martínez-Torrecuadrada J, Míguez-Amil S, Fernández-Leiro R, Blasco M. Journal: STAR protocols - The genetic consequences following polyploidy (i.e., whole-genome duplication; WGD) vary greatly across organisms and through time since polyploidization. At the gene level in allopolyploids, changes include loss/retention of both parental gene
Date: (2023) Authors: Shengchen Shan , Bing Yang , Bernard A. Hauser , Pamela S. Soltis , Douglas E. Soltis Ref: Polyploidy - Posttranscriptional silencing by microRNAs (miRNAs) is a critical constituent of eukaryotic gene regulation. miRNAs are short (~22 nt) noncoding RNAs capable of specifically targeting the miRNA-induced silencing complex (miRISC) to transcripts
Date: (2023) Authors: Qianxin Wu , Yale S. Michaels , Tudor A. Fulga Ref: MicroRNA Detection and Target Identification - Corrigendum: CRISPR-Cas9 Methods and Key Considerations in the Production of Aedes aegypti Mutant Strains. new window
Date: 2024-03-01 Authors: Sun R, Raban R, Akbari OS. Journal: Cold Spring Harbor protocols - The CRISPR–Cpf1 also known as Cas12a is an RNA-guided endonuclease similar to CRISPR–Cas9. Combining the CRISPR–Cpf1 with optogenetics technology, we have engineered photoactivatable Cpf1 (paCpf1) to precisely control the genome sequence in a
Date: (2023) Authors: Takahiro Otabe , Yuta Nihongaki , Moritoshi Sato Ref: Epigenomics - The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes. new window
Date: 2024-03-01 Authors: Boos F, Chen J, Brunet A. Journal: Cold Spring Harbor protocols - The CRISPR-Cas9 system is becoming an imperative tool to edit the genome of various organisms. The gene-editing study by the CRISPR-Cas9 system has revolutionized the diverse field of biomedical research, genome engineering, and gene therapy.
Date: (2023) Authors: Ritesh Kumar , Kartikeya Tiwari , Prakash Saudagar Ref: Gene, Drug, and Tissue Engineering - Quantifying differentiation of progenitor populations using cerebral organoid models for neurodevelopmental disorders. new window
Date: 2024-02-29 Authors: Schroder AL, Fairbanks-Santana M, Rakotomamonjy J, Guemez-Gamboa A. Journal: STAR protocols - The zebrafish is a powerful model system for studying animal development, for modeling genetic diseases, and for large-scale in vivo functional genetics. Because of its ease of use and its high efficiency in targeted gene perturbation, CRISPR–Cas9
Date: (2023) Authors: Saba Parvez , Zachary J. Brandt , Randall T. Peterson Date: 2024-02-23 Authors: Navarro-Guerrero E, Baronio R, Tay C, Knight JC, Ebner DV. Journal: STAR protocols