Results 491-500 of about 1,000
  1. Construction and Evaluation of Zinc Finger Nucleases new window
    Zinc finger nucleases (ZFNs) are programmable nucleases that have contributed significantly to past genome-editing research. They are now utilized much less owing to the advent of transcription activator-like effector nucleases (TALENs) and the
    Date: (2023)
    Authors: Hiroshi Ochiai , Takashi Yamamoto
    Ref: Genome Editing in Animals
    https://experiments.springernature.com/.../978-1-0716-3016-7_1 - SpringerNature
  2. Protocol for detection of in vitro R-loop formation using dot blots. new window
    Date: 2024-01-28
    Authors: Dowling JW, Smith JR, Forero A.
    Journal: STAR protocols
    https://doi.org/10.1016/j.xpro.2024.102857 - Europe PMC
  3. CRISPR Manipulations in Stem Cell Lines new window
    Insights into genome engineering in cells have allowed researchers to cultivate and modify cells as organoids that display structural and phenotypic features of human diseases or normal health status. The generation of targeted mutants is a crucial
    Date: (2023)
    Authors: Ya-Ju Chang , Xuan Cui , Sarah R. Levi , Laura A. Jenny , Stephen H. Tsang
    Ref: Retinitis Pigmentosa
    https://experiments.springernature.com/.../978-1-0716-2651-1_23 - SpringerNature
  4. One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster. new window
    Date: 2024-01-26
    Authors: Xiao Y, Yuan Y, Yadlapalli S.
    Journal: Journal of visualized experiments : JoVE
    https://doi.org/10.3791/64729 - Europe PMC
  5. Genetic Knockout of TE Insertions by CRISPR-Cas9 new window
    Transposable elements (TEs) are abundant in the genome, and specific insertions may be co-opted to act as coding or noncoding functional elements. CRISPR-based genome editing technologies enable functional studies of TE insertions in cell lines.
    Date: (2023)
    Authors: David M. Simpson , Edward B. Chuong
    Ref: Transposable Elements
    https://experiments.springernature.com/.../978-1-0716-2883-6_17 - SpringerNature
  6. Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling c... new window
    Date: 2024-01-11
    Authors: Manske K, Dreßler L, Fräßle SP, Effenberger M, Tschulik C, Cletiu V, Benke E, Wagner M, Schober K, Müller TR, Stemberger C, Germeroth L, Busch DH, Poltorak MP.
    Journal: Journal of immunological methods
    https://doi.org/10.1016/j.jim.2024.113617 - Europe PMC
  7. Combining compact human protein domains with CRISPR systems for robust gene activation new window
    Date: (2023)
    https://www.nature.com/articles/s41592-023-02038-z - SpringerNature
  8. Mouse Gene-Targeting Strategies for Maximum Ease and Versatility. new window
    Date: 2024-01-02
    Authors: Papaioannou VE, Behringer RR.
    Journal: Cold Spring Harbor protocols
    https://doi.org/10.1101/pdb.over107957 - Europe PMC
  9. Functional Analysis of Variants in BRCA1 Using CRISPR Base Editors new window
    To date, methods such as fluorescent reporter assays, embryonic stem cell viability assays, and therapeutic drug-based sensitivity assays have been used to evaluate the function of the variants of uncertain significance (VUS) of the BRCA genes.
    Date: (2023)
    Authors: Ji-Eun See , Yongsub Kim
    Ref: Base Editors
    https://experiments.springernature.com/.../978-1-0716-2879-9_7 - SpringerNature
  10. Recovering a Targeted Mutation in Mice from Embryonic Stem Cell Chimeras or CRISPR-Cas Founders. new window
    Date: 2024-01-02
    Authors: Papaioannou VE, Behringer RR.
    Journal: Cold Spring Harbor protocols
    https://doi.org/10.1101/pdb.over107959 - Europe PMC
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