Results 501-510 of about 1,000
- CRISPR-Cas9-Based Functional Analysis in Amphibians: Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl new windowAmphibians have made many fundamental contributions to our knowledge, from basic biology to biomedical research on human diseases. Current genome editing tools based on the CRISPR-Cas system enable us to perform gene functional analysis in vivo, even
Date: (2023) Authors: Miyuki Suzuki , Midori Iida , Toshinori Hayashi , Ken-ichi T. Suzuki Ref: Genome Editing in Animals Date: 2024-01-02 Authors: Papaioannou VE, Behringer RR. Journal: Cold Spring Harbor protocols - CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor-Resistant Cells new windowEpigenetic alterations have been identified in various tumor types. In part, these alterations are mediated via increased histone deacetylase activity. Although preclinical results of monotherapies with histone deacetylase inhibitors (HDACi) are
Date: (2023) Authors: Paul Jung , Laura Schmalbrock , Matthias Wirth Ref: HDAC/HAT Function Assessment and Inhibitor Development - CRISPR/Cas9 Gene Disruption Studies in F0Xenopus Tadpoles: Understanding Development and Disease in the Frog new windowCRISPR/Cas9 has become the favorite method for gene knockouts in a range of vertebrate model organisms due to its ease of use and versatility. Gene-specific guide RNAs can be designed to a unique genomic sequence and used to target the Cas9
Date: (2023) Authors: Anita Abu-Daya , Annie Godwin Ref: DNA Manipulation and Analysis - Engineering of the mouse germline is a key technology in biomedical research for studying the function of genes in health and disease. Since the first knockout mouse was described in 1989, gene targeting was based on recombination of vector encoded
Date: (2023) Authors: Benedikt Wefers , Wolfgang Wurst , Ralf Kühn Ref: Transgenesis - Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes new windowThe Drosophila ovary represents an outstanding model for investigating tissue homeostasis. Females continuously produce oocytes throughout their lifetime. However, as females age, fecundity declines, in part, due to changes in ovarian niche function
Date: (2023) Authors: Tingting Duan , Felipe Rodriguez-Tirado , Pamela K. Geyer Ref: Drosophila Oogenesis Date: 2024-01-01 Authors: Zhang Y, Qi H, Wang M. Journal: Methods in molecular biology (Clifton, N.J.) - Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression new windowSuspension cells derived from human embryonic kidney cells (HEK 293) are attractive cell lines for retroviral vector production in gene therapeutic development studies and applications. The low-affinity nerve growth factor receptor (NGFR)Nerve growth
Date: (2023) Authors: Stefanie Schatz , Femke Harmina Dijk , Aleksandra Elzbieta Dubiel , Tobias Cantz ... Jörn Stitz Ref: Genotype Phenotype Coupling - Long-branched negatively charged poly(ADP-ribose) (pADPr) is a posttranslation modification of nuclear proteins that play a key role in many chromatin remodeling events. While several enzymes of PARP family could synthesize it across all
Date: (2023) Authors: Yaroslava Karpova , Alexei V. Tulin Ref: Poly(ADP-Ribose) Polymerase - The spatiotemporal control of tissue-specific gene expression is coordinated by cis-regulatory elements (CREs) and associated trans-acting factors. Despite major advances in genome-wide annotation of candidate CREs, the in situ regulatory composition
Date: (2023) Authors: Giovanni A. Botten , Michael Lee , Jian Xu Ref: DNA-Protein Interactions