Results 521-530 of about 1,000
- Engineered SpCas9 variant, SpRY, has been demonstrated to facilitate protospacer adjacent motif (PAM) unrestricted targeting of genomic DNA in various biological systems. Here we describe fast, efficient, and robust preparation of SpRY-derived genome
Date: (2023) Authors: Simon Sretenovic , Xu Tang , Qiurong Ren , Yong Zhang , Yiping Qi Ref: Plant Genome Engineering - Spatial Analysis of Nucleotide Metabolism: From CRISPR Knockout Cancer Cells to MALDI Imaging of Tumors new windowCancer cells depend on nucleotides for proliferation. Inhibition of nucleotide metabolism by antimetabolites is a well-established anticancer therapy. However, resistance and toxicity to antimetabolite treatments reduce their effectiveness. Here, we
Date: (2023) Authors: Petra Hyrossova , Mirko Milosevic , Ahmad Y. Alghadi , Lukas Kucera ... Katerina Rohlenova Ref: Metabolic Reprogramming - Plant Epigenetic Editing to Analyze the Function of Histone Modifications in Gene-Specific Regulation. new window
Date: 2024-01-01 Authors: Oberkofler V, Bäurle I. Journal: Methods in molecular biology (Clifton, N.J.) - Generating genetically modified animal models that precisely recapitulate disease characteristics forms an integral and indispensable tool to understanding disease pathophysiology. Recently, important advances in genome editing technologies have
Date: (2023) Authors: Hiroki Sasaguri Ref: Genome Editing in Animals - Optimized Protocol for the Regulation of DNA Methylation and Gene Expression Using Modified dCas9-SunTag Platforms. new window
Date: 2024-01-01 Authors: Morita S, Horii T, Hatada I. Journal: Methods in molecular biology (Clifton, N.J.) - Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacter... new windowMedicago truncatula is the model plant species for studying symbioses with nitrogen-fixing rhizobia and arbuscular mycorrhizae, where edited mutants are invaluable for elucidating the contributions of known genes in these processes. Streptococcus
Date: (2023) Authors: Tom Lawrenson , Nicola Atkinson , Macarena Forner , Wendy Harwood Ref: Plant Genome Engineering Date: 2024-01-01 Authors: Hamilton PJ, Lim CJ, Nestler EJ, Heller EA. Journal: Methods in molecular biology (Clifton, N.J.) - Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice new windowBase editing technology is a modified CRISPR/Cas system that directly introduces point mutations into targeted genomic regions without causing double-stranded DNA breaks. Target-AID (activation-induced cytidine deaminase) is a representative base
Date: (2023) Authors: Hiroki Sasaguri Ref: Base Editors Date: 2024-01-01 Authors: Kim SH, Haynes KA. Journal: Methods in molecular biology (Clifton, N.J.) - Analysis of Neutrophil Morphology and Function Under Genetic Perturbation of Transcription Factors In Vitro new windowHoxb8 cells are immortalized myeloid progenitors that maintain their multipotent potential and can be differentiated into neutrophils. Genetic modification of Hoxb8 cells can be used as a model system for the functional analysis of regulators of
Date: (2023) Authors: Julia Salafranca , Zhichao Ai , Lihui Wang , Irina A. Udalova , Erinke Grinsven Ref: Transcription Factor Regulatory Networks