Results 541-550 of about 1,000
- Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System. new window
Date: 2024-01-01 Authors: Chakraborty M, Powichrowski J, Bruder MR, Nielsen L, Sung C, Boegel SJ, Aucoin MG. Journal: Methods in molecular biology (Clifton, N.J.) - The CRISPR/Cas9 system is an extremely powerful tool for targeted mutagenesis in plants. However, plant genome editing relies on the labor-intensive plant regeneration method for generating gene-edited plants. To overcome this bottleneck, several
Date: (2023) Authors: Hyeonjin Kim , Youngbin Oh , Eunae Park , Moonyoung Kang ... Sang-Gyu Kim Ref: Base Editors - Rapid Gene Silencing Followed by Antimicrobial Susceptibility Testing for Target Validation in Antibiotic Discovery. new window
Date: 2024-01-01 Authors: Daniel C, Willcocks S, Bhakta S. Journal: Methods in molecular biology (Clifton, N.J.) - DNA base editors, one of the CRISPR-based genome editing tools, can induce targeted point mutations at desired sites. Their superiority is based on the fact that they can perform efficient and precise gene editing without generating a DNA
Date: (2023) Authors: Beomjong Song , Sangsu Bae Ref: Base Editors - Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production. new window
Date: 2024-01-01 Authors: Inwood S, Cheng K, Betenbaugh MJ, Shiloach J. Journal: Methods in molecular biology (Clifton, N.J.) - CRISPR systems can be leveraged to direct recombinases to integrate gene-sized DNA sequences.
Date: (2023) Authors: Lei Tang - Generation of conditional knockout mice using the Cre-loxP system is essential for the analysis of gene functions. The use of CRISPR-Cas9 in combination with two sets of guide RNAs and single-stranded oligonucleotides including loxP sites enables
Date: (2023) Authors: Takuro Horii , Ryosuke Kobayashi , Izuho Hatada Ref: Genome Editing in Animals - Precise and Scarless Insertion of Transposable Elements by Cas9-Mediated Genome Engineering new windowTransposable element insertions can have broad effects on gene expression, ranging from new regulatory functions to pathogenic consequences by transplanting new cis-regulating elements or perturbing existing ones. Genetic manipulation of such DNA
Date: (2023) Authors: Vivien Marie Weber , Aurélien J. Doucet , Gael Cristofari Ref: Transposable Elements - CRISPR base editors are genome-modifying proteins capable of creating single-base substitutions in DNA but without the requirement for a DNA double-strand break. Given their ability to precisely edit DNA, they hold tremendous therapeutic potential.
Date: (2023) Authors: Colin K. Lim , Angelo J. Miskalis , Pablo Perez-Pinera , Thomas Gaj Ref: Base Editors Date: 2024-01-01 Authors: Poot M. Journal: Methods in molecular biology (Clifton, N.J.)