Results 561-570 of about 1,000
Date: 2024-01-01 Authors: Ahmad N, Fatima S, Hundleby P, Mehboob-Ur-Rahman. Journal: Methods in molecular biology (Clifton, N.J.) - Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade–Cas3 system new windowA lack of generic and effective genetic manipulation methods for Pseudomonas has restricted fundamental research and utilization of this genus for biotechnology applications. Phage-encoded homologous recombination (PEHR) is an efficient tool for
Date: (2023) Authors: Wentao Zheng , Yandong Xia , Xue Wang , Shiqing Gao ... Jia Yin - Our ability to modify DNA molecules and to introduce them into mammalian cells or embryos almost appears in parallel, starting from the 1970s of the last century. Genetic engineering techniques rapidly developed between 1970 and 1980. In contrast,
Date: (2023) Authors: Lluis Montoliu Ref: Transgenesis Date: 2024-01-01 Authors: Freudhofmaier M, Hoyle JW, Maghuly F. Journal: Methods in molecular biology (Clifton, N.J.) - Knowledge gaps persist on signaling pathways and metabolic states in germ cells sufficient to support spermatogenesis independent of a somatic environment. Consequently, methods to culture mammalian stem cells through spermatogenesis in defined
Date: (2023) Authors: Karen M. Chapman , Ashutosh Pudasaini , Morgan N. Vanderbeck , F. Kent Hamra Ref: Germline Stem Cells Date: 2024-01-01 Authors: Freudhofmaier M, Hoyle JW, Maghuly F. Journal: Methods in molecular biology (Clifton, N.J.) - Homology-directed repair (HDR), a method for repair of DNA double-stranded breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA donors, but remains limited by low efficiency and off-target effects. In this
Date: (2023) Authors: Stephan Riesenberg , Philipp Kanis , Dominik Macak , Damian Wollny ... Svante Pääbo - The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor. Here we describe a set of protocols for using the catalytically inactive dead Cas9 (dCas9)-based tools, including the bipartite super repressor
Date: (2023) Authors: Nan Cher Yeo , George M. Church Ref: Transcription Factor Regulatory Networks - CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272). new window
Date: 2024-01-01 Authors: Rojas-Vásquez R, Hernández-Soto A, Arrieta-Espinoza G, Gatica-Arias A. Journal: Methods in molecular biology (Clifton, N.J.) - While the zebrafish has for some time been regarded as a powerful model organism with which to study early events in hematopoiesis, recent evidence suggests that it also ideal for unraveling the molecular requirements for T cell development in the
Date: (2023) Authors: Robert Sertori , Yong Zhang , David L. Wiest Ref: T-Cell Development