Results 701-710 of about 1,000
- Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster new windowThe Notch gene is a key factor in the signaling cascade that allows communication between neighboring cells in many organisms, from worms and insects to humans. The relative simplicity of the Notch pathway in Drosophila, combined with a powerful set
Date: (2022) Authors: Oleg V. Andreyenkov , Elena I. Volkova , Natalya G. Andreyenkova , Sergey A. Demakov Ref: Notch Signaling Research - Protocol for editing fibroblasts with in vitro transcribed Cas9 mRNA and profile off-target editing by optimized GUIDE-s... new window
Date: 2023-10-27 Authors: Li Z, Reint G, Haapaniemi EM. Journal: STAR protocols - CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells new windowNatural killer (NK) cells are an important component of the cancer immune surveillance system. They are regulated by germline-encoded receptors that activate and inhibit their effector function, such as secretion of cytokines and direct lysis of
Date: (2022) Authors: Xiaoxuan Zhuang , Eric O. Long Ref: Natural Killer (NK) Cells - Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping: A pipeline for whole-brain structural and activity ana... new window
Date: 2023-10-26 Authors: Jin DS, Neelakantan U, Lacadie CM, Chen T, Rooney B, Liu Y, Wu W, Wang Z, Papademetris X, Hoffman EJ. Journal: STAR protocols - Forward genetic screens across hundreds of cancer cell lines have started to define the genetic dependencies of proliferating human cells. However, most such screens have been performed in vitro with little consideration into how medium composition
Date: (2022) Authors: Kimberly S. Huggler , Nicholas J. Rossiter , Kyle M. Flickinger , Jason R. Cantor Ref: Essential Genes and Genomes - Protocol for establishing a protein interactome based on close physical proximity to a target protein within live buddin... new window
Date: 2023-10-25 Authors: Kolhe JA, Babu NL, Freeman BC. Journal: STAR protocols - A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria new windowCyanobacteria, a group of diverse bacteria capable of oxygenic photosynthesis, are excellent models for investigating many important cellular processes, such as photosynthesis, nitrogen fixation, and prokaryotic cell differentiation. They also have
Date: (2022) Authors: Ju-Yuan Zhang , Tian-Cai Niu , Gui-Ming Lin , Cheng-Cai Zhang Ref: Essential Genes and Genomes - Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors. new window
Date: 2023-10-20 Authors: Benyamini B, Esbin MN, Whitney O, Walther N, Maurer AC. Journal: Journal of visualized experiments : JoVE - CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pasto... new windowEfficient targeted genome engineering of Komagataella phaffii requires balanced expression of Cas9 nuclease and a target-specific guide RNA (gRNA). In addition, correct processing of the transcribed RNA to provide the designed gRNA as a target
Date: (2022) Authors: Jasmin Elgin Fischer , Anton Glieder Ref: Yeast Metabolic Engineering - Protocol for auxin-inducible depletion of the RNA-binding protein PTBP1 in mouse embryonic stem cells. new window
Date: 2023-10-19 Authors: Kainov Y, Zhuravskaya A, Makeyev EV. Journal: STAR protocols