Results 191-200 of about 359
- Embryogenesis is a tightly coordinated process that involves elongation to form the antero–posterior (AP) axis of the body and formation of somites and distinct sensorimotor circuits. While in vitro systems recapitulating somitogenesis have been
Date: (2023) Authors: Madhura Mukhopadhyay - The first step in single-cell RNAseq data analysis is the evaluation of the overall quality of the cell transcriptome and the preparation of the single-cell transcription data for clustering. In this chapter, we describe one of the possible
Date: (2023) Authors: Martina Olivero , Raffaele A. Calogero Ref: Single Cell Transcriptomics - Statistical Analysis of Multiplex Immunofluorescence and Immunohistochemistry Imaging Data new windowAdvances in multiplexed single-cell immunofluorescence (mIF) and multiplex immunohistochemistry (mIHC) imaging technologies have enabled the analysis of cell-to-cell spatial relationships that promise to revolutionize our understanding of
Date: (2023) Authors: Julia Wrobel , Coleman Harris , Simon Vandekar Ref: Statistical Genomics - Visualizing the Interactions Shaping the Imaging of the Microenvironment in Human Cancers new windowThe Visium Spatial Gene Expression Solution (Visium 10×) allows for the mRNA analysis using high throughput sequencing and maps a transcriptional expression pattern in tissue sections using high-resolution microscope imaging in ex-vivo human and mice
Date: (2023) Authors: Antonio G. Solimando , Vanessa Desantis , Matteo Claudio Vià Ref: Tumor Angiogenesis Assays - Magnetic resonance imaging (MRI) is a noninvasive imaging technique that allows for physiological and functional studies of the tumor microenvironment. Within MRI, the emerging field of chemical exchange saturation transfer (CEST) has been largely
Date: (2023) Authors: Dario Livio Longo , Elisa Pirotta , Riccardo Gambino , Feriel Romdhane ... Alessia Corrado Ref: The Tumor Microenvironment - Proteins are the functional molecules for almost all cellular and biological processes. They are also the targets of most drugs. Proteins employ complex, multilevel regulations, so their abundance levels do not well correlated with their mRNA
Date: (2023) Authors: Xiaoyu Song Ref: Statistical Genomics - A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues new windowThe neurovascular unit (NVU), composed of endothelial cells, pericytes, juxtaposed astrocytes and microglia together with neurons, is essential for proper central nervous system functioning. The NVU critically regulates blood–brain barrier (BBB)
Date: (2023) Authors: Daniel Spitzer , Maryam I. Khel , Tim Pütz , Jenny Zinke ... Kavi Devraj - Working with Omics Data: An Interdisciplinary Challenge at the Crossroads of Biology and Computer Science new windowNowadays, generating omics data is a common activity for laboratories in biology. Experimental protocols to prepare biological samples are well described, and technical platforms to generate omics data from these samples are available in most
Date: (2023) Authors: Thibault Poinsignon , Pierre Poulain , Mélina Gallopin , Gaëlle Lelandais Ref: Machine Learning for Brain Disorders - Standardization of Single-Cell RNA-Sequencing Analysis Workflow to Study Drosophila Ovary new windowDevelopments in single-cell technology have considerably changed the way we study biology. Significant efforts have been made over the last few years to build comprehensive cell-type-specific transcriptomic atlases for a wide range of tissues in
Date: (2023) Authors: Deeptiman Chatterjee , Wu-Min Deng Ref: Germline Stem Cells - Although the subcellular dynamics of RNA and proteins are key determinants of cell homeostasis, their characterization is still challenging. Here we present an integrative framework to simultaneously interrogate the dynamics of the transcriptome and
Date: (2023) Authors: Eneko Villanueva , Tom Smith , Mariavittoria Pizzinga , Mohamed Elzek ... Kathryn S. Lilley